This kit is further developed based on the principle of efficient and rapid connection of DNA fragments by topoisomerase. Compared with the traditional T4 ligase, it has the following advantages: 1) fast, and the connection reaction can be completed within only 1-5 min. 2) High efficiency, no self-connection, the positive cloning rate is close to 100%, and there is no need to set up blue and white spot screening; 3) The operation is simple, and it takes only 15-20 min from connecting to the coating plate. Ice bath, heat shock and 1 hour resuscitation are omitted in the operation process. 4) It can connect up to 5 kb of products.
Product type |
Cloning Kit |
Antibiotic-resistant Bacteria |
Ampicillin (AmpR) |
Methods of cloning |
TA |
Conditions of carriage |
Dry ice |
Components No. |
Name |
10907ES20 (20T) |
10907ES50 (50T) |
10907-A |
pESI-T vector (30 ng/μL) |
20 μL |
50 μL |
10907-B |
1 kb control insert (40 ng/μL) |
5 μL |
5 μL |
10907-C |
10× Enhancer |
20 μL |
50 μL |
The product should be stored at -25°C~-15℃ for 1 year.
Ver.EN20230915
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