Product Information

 

 

 

Product Name	Cat#	Specification
Hieff NGS® Ultima Dual-mode RNA Library Prep Kit for Illumina®	12252ES24	24 rxn
	12252ES96	96 rxn

Product Description

 

Hieff NGS™ Ultima Dual-mode RNA Library Prep Kit for Illumina™ is an RNA sequencing library construction kit for the Illumina™ sequencing platform, including RNA fragmentation reagents, reverse transcription reagents, conventional and strand-specific ds-cDNA synthesis reagents, and library amplification reagents. The sequencing library can be constructed by connecting the mRNA purification kit or rRNA removal kit. The two-strand synthesis module is equipped with two buffers, and customers can build a library according to their needs. Among them, dTTP is replaced with dUTP in the strand-specific two-strand synthesis Buffer, so that dUTP is incorporated into the second strand of cDNA, and the high-fidelity DNA polymerase used in this kit cannot amplify the DNA template containing uracil, achieving strand specificity. All reagents provided have undergone strict quality control and functional verification, ensuring the stability and reproducibility of library construction to the greatest extent.

 

Shipping and Storage

 

All the components are shipped with dry ice and can be stored at -20°C for one year.

Cautions

1.1 For your safety and health, please wear lab coats and disposable gloves for operation.

1.2 Thaw components at room temperature. Once the components are thawed, mix thoroughly by vortexing, spin the tube briefly and place on ice for later use.

1.3 It is recommended to perform each reaction step in a thermocycler with a heated lid. The thermocycler should be preheated to the set temperature before use.

1.4 The PCR product is prone to aerosol pollution due to improper operation, which will affect the accuracy of the experimental results. It is recommended to physically separate the PCR reaction preparation area and PCR product purification-detection area, use special pipettes and other equipment and clean the experimental area regularly.

 

1.5 For research use only!

 

 

Operation flowchart

Figure 1  RNA library construction flowchart