This product is connected to Hieff NGSTM Ultima Dual-mode RNA Library Prep Kit for Illumina® (Cat#12252) or Hieff NGSTM Ultima Dual-mode RNA Library Prep Kit for MGI® (Cat#13333), replaces Frag/ Prime buffer which is used for RNA fragmentation and one-strand cDNA synthesis steps. Input RNA can be a total RNA or a product of mRNA purification or rRNA removal, etc. Since the RNA from the mRNA purification or rRNA removal is directly eluted with 1× Frag/Prime Buffer in Cat#12252 or Cat#13333, there is no additional volume for adding RNA-containing solutions. If the input RNA has been dissolved in Nuclease free ddH2O, this product can be used for RNA fragmentation or one-strand cDNA synthesis.
All the components are shipped with dry ice and can be stored at -20°C for one year.
1. For your safety and health, please wear lab coats and disposable gloves for operation.
2. For research use only!