Hieff NGS™ MaxUp rRNA Depletion Kit (Plant) is designed to remove rRNA (including cytoplasmic 18S and 25S rRNA, mitochondrial 18S and 26S rRNA, chloroplast 16S and 23S rRNA) from plant total RNA based on RNase H-based workflow and to retain mRNA and other non-coding RNA. This kit is suitable for both intact and partially degraded RNA samples.
|Depletion Technology||RNase H|
|Sample Type||Total RNA of Plant|
|Final Product Type||mRNA and other non coding RNAs|
|No. of Reactions||24/96 Preps|
|Starting Material Amount||100 ng~1 μg total RNA|
|12254-A||Hybridization Buffer||72 μL||288 μL|
|12254-B||Probe Mix (Plant)||72 μL||288 μL|
|12254-C||RNase H Buffer||72 μL||288 μL|
|12254-D||RNase H||48 μL||192 μL|
|12254-E||DNase I Buffer||660 μL||2×1320 μL|
|12254-F||DNase I||60 μL||240 μL|
-25℃ ~ -15℃ storage, valid for one year.
Figure1. High deletion rate in various plant using Hieff NGS™ MaxUp rRNA Depletion Kit (Plant).
1 μg Total RNA from various plant was used and RNA library was constructed using YEASEN Dual-mode RNA Library Prep Kit (Cat#12308) and rRNA Depletion Kit (Cat#12254). Calculated the percent reads of undeletion and deletion rate of rRNA rate in these RNA library.