Taq DNA polymerase and its antibody are two key factors of hot start PCR, which is a technique for improving the target amplification product in a PCR process while preventing non-target amplification. Primer dimer-induced mismatch or non-specific amplification can be significantly reduced by using Taq DNA polymerase antibody inhibiting enzyme activity, which can render it inactive at low temperatures, denatured when heated to denaturing temperature, deblocking, and therefore releasing activity.