Description

2×Hieff^TM PCR Master Mix is a kind of conventional PCR premixed solution which is ready to use, including Hieff^TM Taq DNA Polymerase (Cat#10101), dNTP mix, MgCl₂ and optimized buffer. During the reaction, only the primer and template can be added for amplification, which greatly simplifies the operation steps of experiment. This product contains excellent stabilizers and can be stored for 3 months at 4℃. The PCR product have 3 '-dA protrusion and can be easily cloned into T vector.

Features

• Convenient, ready-to-use mix
• Thermostable—half-life is more than 40 min at 95℃
• Generates PCR products with 3'-dA overhangs

Applications

• Routine PCR amplification of DNA fragments
• High throughput PCR

Specifications

Fidelity (vs. Taq)	1×
Hot Start	No
Overhang	3 '-A
Polymerase	Taq DNA Polymerase
Reaction Format	SuperMix or Master Mix
Reaction Speed	Standard
Product Type	PCR Master Mix (2x)

Storage

The 2×Hieff^TM PCR Master Mix products should be stored at -25~-15℃ for 2 years.

Figures

2×Hieff^™ PCR master mix amplification result

Figure 1 The expected 1.2 kb PCR products can be amplified with 2×Hieff^TM PCR Master Mix.

The Master Mix was stored at -20℃ for 1 year following another 3 months at 4℃ and 1 month at 25℃. Template: Arabidopsis genome. Annealing temperature: 60℃. Extension time: 40 sec.

Ver.EN20230901