Products in stock in the United States can be delivered within 2 days.
+1-2404726069 (U.S.) +86-027-65528241 (Outside U.S.) 
overseas@yeasen.com shop website:https://yeabio.com
0Hifair™ III 1st Strand cDNA Synthesis Kit (gDNA digester plus)
Product Description
HifairTM III 1st Strand cDNA Synthesis Kit (gDNA digester plus) is a first-strand synthesis kit of cDNA that contains gDNA removal steps. The kit was developed based on HifairTM III Reverse Transcriptase. The enzyme cDNA synthesis speed is fast, the thermal stability is greatly improved, can withstand reaction temperatures up to 60°C, suitable for reverse transcription of RNA templates with complex secondary structure. At the same time, the enzyme enhances the affinity with the template, suitable for a small number of templates and reverse transcription of low-copy genes. HifairTM III Reverse Transcriptase's ability to synthesize full-length cDNA has also been improved, with a synthesis of up to 19.8 kb cDNA.
The kit contains gDNA digester Mix, which digests residual genomic DNA contamination from the RNA template, ensuring more reliable subsequent results. The kit offers two cDNA synthesis primers: Random Primers N6 and Oligo (dT)18, with the user's choice of Random as needed Primers N6, Oligo (dT)18 or Gene Specific Primers as reverse transcription primers, synthesized single-stranded cDNA The product can be used directly for subsequent PCR or qPCR reactions.
Shipping and Storage
The product is shipped with ice packs and can be stored at -20℃ for 18 months.
Cautions
1. The preparation of the reaction solution should be completed on ice, and the operation process should avoid RNase contamination.
2. It is recommended that the RNA is soluble in water rather than TE, as TE interferes with gDNA removal as well as reverse transcription.
3. 5×gDNA digester Mix、10×HifairTM III Super Buffer、HifairTM III RT Enzyme Mix gently upside down and carefully centrifuge to the bottom before use. Use a pipette with the right range when aspirating liquid, and do not insert the tip too deep under the liquid level.
4. qPCR experiments recommend genomic removal steps to ensure that the results are more realistic and reliable.
5. This product is for research use ONLY！
On the selection of reverse transcription primers
1. If the template is of eukaryotic origin, it is recommended to choose Oligo (dT)18, paired with the 3' PolyA tail of eukaryotic mRNA, to obtain the maximum yield of full length cDNA.
2. Reverse transcription of prokaryote RNA should use Random Primers N6 or gene-specific primers.
3. Random Primers N6 is widely applicable to mRNA, rRNA, tRNA, small RNA and LncRNA plates.
[1] Ba F, Liu Y, Liu WQ, Tian X, Li J. SYMBIOSIS: synthetic manipulable biobricks via orthogonal serine integrase systems. Nucleic Acids Res. 2022;50(5):2973-2985. doi:10.1093/nar/gkac124(IF:16.971)
[2] Zhang Y, Ding H, Wang X, et al. MK2 promotes Tfcp2l1 degradation via β-TrCP ubiquitin ligase to regulate mouse embryonic stem cell self-renewal. Cell Rep. 2021;37(5):109949. doi:10.1016/j.celrep.2021.109949(IF:9.423)
[3] Liu X, Zhan T, Gao Y, et al. Benzophenone-1 induced aberrant proliferation and metastasis of ovarian cancer cells via activated ERα and Wnt/β-catenin signaling pathways. Environ Pollut. 2022;292(Pt B):118370. doi:10.1016/j.envpol.2021.118370(IF:8.071)
[4] Li C, Song J, Guo Z, et al. EZH2 Inhibitors Suppress Colorectal Cancer by Regulating Macrophage Polarization in the Tumor Microenvironment. Front Immunol. 2022;13:857808. Published 2022 Apr 1. doi:10.3389/fimmu.2022.857808(IF:7.561)
[5] Wu Z, Lu Z, Li L, et al. Identification and Validation of Ferroptosis-Related LncRNA Signatures as a Novel Prognostic Model for Colon Cancer. Front Immunol. 2022;12:783362. Published 2022 Jan 26. doi:10.3389/fimmu.2021.783362(IF:7.561)
[6] Zhang Y, Hu R, Xi B, Nie D, Xu H, Liu A. Mechanisms of Senescence-Related NKG2D Ligands Release and Immune Escape Induced by Chemotherapy in Neuroblastoma Cells. Front Cell Dev Biol. 2022;10:829404. Published 2022 Mar 2. doi:10.3389/fcell.2022.829404(IF:6.684)
[7] Zheng XR, Zhang MJ, Qiao YH, et al. Cyclocarya paliurus Reprograms the Flavonoid Biosynthesis Pathway Against Colletotrichum fructicola. Front Plant Sci. 2022;13:933484. Published 2022 Jun 30. doi:10.3389/fpls.2022.933484(IF:6.627)
[8] Tian Q, Zhou LQ. Lactate Activates Germline and Cleavage Embryo Genes in Mouse Embryonic Stem Cells. Cells. 2022;11(3):548. Published 2022 Feb 4. doi:10.3390/cells11030548(IF:6.600)
[9] Zhang W, Lin J, Li J, et al. Rambutan genome revealed gene networks for spine formation and aril development. Plant J. 2021;108(4):1037-1052. doi:10.1111/tpj.15491(IF:6.486)
[10] Fu S, Tan R, Feng Y, et al. N-methyl-N-nitrosourea induces zebrafish anomalous angiogenesis through Wnt/β-catenin pathway [published online ahead of print, 2022 May 25]. Ecotoxicol Environ Saf. 2022;239:113674. doi:10.1016/j.ecoenv.2022.113674(IF:6.291)
[11] Wang G, Wang X, Ma H, et al. PcWRKY11, an II-d WRKY Transcription Factor from Polygonum cuspidatum, Enhances Salt Tolerance in Transgenic Arabidopsis thaliana. Int J Mol Sci. 2022;23(8):4357. Published 2022 Apr 14. doi:10.3390/ijms23084357(IF:5.924)
[12] Xing YJ, Liu BH, Wan SJ, et al. A SGLT2 Inhibitor Dapagliflozin Alleviates Diabetic Cardiomyopathy by Suppressing High Glucose-Induced Oxidative Stress in vivo and in vitro. Front Pharmacol. 2021;12:708177. Published 2021 Jul 12. doi:10.3389/fphar.2021.708177(IF:5.811)
[13] Wei MP, Yu H, Guo YH, Cheng YL, Xie YF, Yao WR. Synergistic antibacterial combination of Sapindoside A and B changes the fatty acid compositions and membrane properties of Cutibacterium acnes [published online ahead of print, 2021 Nov 19]. Microbiol Res. 2021;255:126924. doi:10.1016/j.micres.2021.126924(IF:5.415)
[14] Li N, Chen J, Geng C, et al. Myoglobin promotes macrophage polarization to M1 type and pyroptosis via the RIG-I/Caspase1/GSDMD signaling pathway in CS-AKI. Cell Death Discov. 2022;8(1):90. Published 2022 Feb 28. doi:10.1038/s41420-022-00894-w(IF:5.241)
[15] Zhang Q, Yang R, Tian Y, et al. Ribavirin inhibits cell proliferation and metastasis and prolongs survival in soft tissue sarcomas by downregulating both protein arginine methyltransferases 1 and 5. Basic Clin Pharmacol Toxicol. 2022;131(1):18-33. doi:10.1111/bcpt.13736(IF:4.084)
[16] Wang PT, Li N, Wang XY, et al. RIG-I, a novel DAMPs sensor for myoglobin activates NF-κB/caspase-3 signaling in CS-AKI model. Mil Med Res. 2021;8(1):37. Published 2021 Jun 21. doi:10.1186/s40779-021-00333-4(IF:3.329)
[17] Peng G, Zhu C, Sun Q, et al. Testicular miRNAs and tsRNAs provide insight into gene regulation during overwintering and reproduction of Onychostoma macrolepis. Fish Physiol Biochem. 2022;48(3):481-499. doi:10.1007/s10695-022-01078-0(IF:2.794)
[18] Ye Z, Xu YJ, Liu Y. Influence of different dietary oil consumption on nutrient malabsorption: An animal trial using Sprague Dawley rats. J Food Biochem. 2021;45(4):e13695. doi:10.1111/jfbc.13695(IF:2.720)
[19] Li Y, Qin G, Du J, Yue P, Zhang Y, Hou N. circRNA LDLRAD3 Enhances the Malignant Behaviors of NSCLC Cells via the miR-20a-5p-SLC7A5 Axis Activating the mTORC1 Signaling Pathway. J Healthc Eng. 2022;2022:2373580. Published 2022 Jan 6. doi:10.1155/2022/2373580(IF:2.682)
[20] Peng G, Sun Q, Chen Y, et al. A comprehensive overview of ovarian small non-coding RNAs in the late overwintering and breeding periods of Onychostoma macrolepis. Comp Biochem Physiol Part D Genomics Proteomics. 2022;42:100967. doi:10.1016/j.cbd.2022.100967(IF:2.674)
[21] Xu Y, Peng W, Han D, et al. Maiwei Yangfei decoction prevents bleomycin-induced pulmonary fibrosis in mice. Exp Ther Med. 2021;22(5):1306. doi:10.3892/etm.2021.10741(IF:2.447)
Catalog No.:*
Name*
phone Number:*
Lot:*
Email*
Country:*
Company/Institute:*
