Hieff™ Gold T4 DNA Ligase can catalyze the formation of phosphodiester bonds at the 5' phosphate end and 3' hydroxyl end of adjacent nucleic acids at blunt or sticky ends of dsDNA when ATP is used as a coenzyme, and can also catalyze RNA to link with ssDNA or ssRNA in double strands, but cannot catalyze linkages between fully single-stranded nucleotides.
This product is suitable for labeling the 3'-end of RNA, circularizing RNA and DNA oligonucleotides, cloning cDNA, etc.
|Product Type||T4 DNA Ligase|
|10300-A||Hieff™ Gold T4 DNA Ligase (5 U/µL)||200 µL|
|10300-B||10×Ligase Buffer||400 µL|
Figure 1: Ligation of Lambda DNA digest using Hieff Gold T4 DNA ligase
The product should be stored at -25°C~-15°C for two years. Please avoid repeated freeze-thaw.