Hieff Unicon™ Hotstart Fast Taq DNA polymerase, 5 U/μL

Hieff Unicon™ Hotstart Fast Taq DNA polymerase is a thermostable Taq DNA Polymerase mutant complexed with a proprietary antibody that inhibits polymerase activity below 60℃. The polymerase activity can only be released after heating at 95℃, thus avoiding nonspecific amplification and primer-dimer formation during sample preparation and heating process prior to PCR cycling. Hieff Unicon Hotstart Fast Taq DNA polymerase, like wild-type Taq DNA polymerase, also has 5′-3′ polymerase activity and 5′-3′ exonuclease activity, but lacks any detectable 3′-5′ exonuclease activity.

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Product Information

Product name

Cat#

Size

10723ES72

250 U

10723ES76

500 U

Hieff UniconTM Hotstart Fast Taq DNA polymerase, 5 U/μL

10723ES80

1000 U

10723ES92

10000 U

10723ES93

25000 U

Product Description

Hieff UniconTM Hotstart Fast Taq DNA polymerase is a thermostable Taq DNA Polymerase mutant complexed with a proprietary antibody that inhibits polymerase activity below 60. The polymerase activity can only be released after heating at 95, thus avoiding nonspecific amplification and primer-dimer formation during sample preparation and heating process prior to PCR cycling. Hieff UniconTM Hotstart Fast Taq DNA polymerase, like wild-type Taq DNA polymerase, also has 5′-3′ polymerase activity and 5′-3′ exonuclease activity, but lacks any detectable 3′-5′ exonuclease activity.

Package Information

Component number

Component

Cat#/Size

10723ES72

(250 U)

10723ES76(500 U)

10723ES80(1000 U)

10723ES92(10000 U)

10723ES93(25000 U)

10723

Hotstart Fast Taq (5 U/μL)

50 μL

100 μL

200 μL

1 mL×2

1 mL×5

Shipping and Storage

The product is shipped with ice packs and can be stored at -20°C for 2 years.

Reaction System

Components

Vloume(μL

Final concentration

2×Buffer

10

1×

Primer mix (10 μmol/L)

0.2-1

0.1 μmol/L-0.5 μmol/L

Probe mix (10 μmol/L)

0.2-1

0.1 μmol/L-0.5 μmol/L

Hotstart Fast Taq (5 U/μL)

0.5

2.5 U

DNA Template

100 pg-100 ng

-

Sterile ultra-pure water

up to 20

-

[Note]: The optimal concentration for above components can be adjusted according to the specific experimental conditions.

Amplification Procedure (Two-Step Method)

Cycle step

Temperature

Time

Cycles

Initial denaturation

95℃

5 min

1

denaturation

annealing/extension

95℃

60℃

15 sec

30 sec

40

[Note]: The annealing/extension temperature can be appropriately adjusted according to the experimental requirements.

Caution

For your safety and health, please wear lab coats and disposable gloves for operation.