Product Description

Hieff UNICON^™ Hotstart J-Taq DNA polymerase is a thermostable Taq DNA Polymerase mutant complexed with a proprietary antibody that inhibits polymerase activity below 60℃. The polymerase activity can only be released after heating at 95℃, thus avoiding nonspecific amplification and primer-dimer formation during sample preparation and heating process prior to PCR cycling. Hieff UNICON^™ Hotstart J-Taq DNA polymerase, like wild-type Taq DNA polymerase, also has 5′-3′ polymerase activity and 5′-3′ exonuclease activity but lacks any detectable 3′-5′ exonuclease activity.

Package Information

Component number	Component	Cat#/Size
		10723ES72(250 U)	10723ES76(500 U)	10723ES80(1000 U)	10723ES92(10000 U)	10723ES93(25000 U)
10723	Hotstart J-Taq (5 U/μL)	50 μL	100 μL	200 μL	1 mL×2	1 mL×5

Shipping and Storage

The product is shipped with ice packs and can be stored at -20°C for 2 years.

Reaction System

Components	Vloume（μL）	Final concentration
2×Buffer	10	1×
Primer mix (10 μmol/L)	0.2-1	0.1 μmol/L-0.5 μmol/L
Probe mix (10 μmol/L)	0.2-1	0.1 μmol/L-0.5 μmol/L
Hotstart J-Taq (5 U/μL)	0.5	2.5 U
DNA Template	100 pg-100 ng	-
Sterile ultra-pure water	up to 20	-

[Note]: The optimal concentration for above components can be adjusted according to the specific experimental conditions.

Amplification Procedure (Two-Step Method)

Cycle step	Temperature	Time	Cycles
Initial denaturation	95℃	5 min	1
denaturation annealing/extension	95℃ 60℃	15 sec 30 sec	40

[Note]: The annealing/extension temperature can be appropriately adjusted according to the experimental requirements.

Caution

For your safety and health, please wear lab coats and disposable gloves for operation.