Hifair™ IV Reverse Transcriptase is a new reverse transcriptase based on Hieff™ M-MLV (H-) Reverse Transcriptase. The enzyme possesses high thermal stability(withstand 60°C), which is suitable for reverse transcription of RNA templates with complex secondary structures compared with Hieff™ M-MLV (H-) Reverse Transcriptase. Hifair™ IV Reverse Transcriptase enhances the affinity with the template, which is suitable for reverse transcription and mRNA library preparation of low-copy genes. The ability of Hifair™ IV Reverse Transcriptase to synthesize full-length cDNA has also been improved which enable the enzyme to amplify cDNA up to 10 kb.

Applications

preparation of full-length cDNA library; end-point PCR; real-time quantitative PCR, etc.

Shipping and Storage

All the components are shipped with ice packs and can be stored at -20°C for one year.

Cautions

1) Please keep the experimental area clean; wear clean gloves and masks during operation; all consumables used in the experiment must be RNase free to prevent RNase contamination;

2) All operations should be performed on ice to prevent RNA degradation;

3) To ensure the success of reverse transcription, it is recommended to use high-quality RNA samples;

4) For your safety and health, please wear a lab coat and disposable gloves for operation.

5) For research use only!