Product description

Hifair™ Ⅲ Reverse Transcriptase is an updated version of Hifair™ Ⅱ Reverse Transcriptase obtained through genetic engineering technology. It has higher cDNA synthesis ability and speed, thermal stability and reaction temperature limit (up to 60°C) than Hifair™ Ⅱ Reverse Transcriptase. The synthesized cDNA product is up to 19.8 kb. Hifair™ Ⅲ Reverse Transcriptase enhances the affinity of the templates and is suitable for reverse transcription of RNA templates with complex secondary structure or low copy genes.

Specifications

Cat#	11111ES92/11111ES93/11111ES08
Size	10,000 U/50,000 U/ 5 mL
Unit Definition	One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37°C using Oligo(dT) as primers.

Components

Components No.	Name	11111ES92	11111ES93	11111ES08
11111-A	5×HifairTM Ⅲ Buffer	250 μL	1,250 μL	25 mL
11111-B	HifairTM Ⅲ Reverse Transcriptase (200 U/μL)	50 μL	250 μL	5 mL

Storage

The product should be stored at -25℃ ~ -15℃ for 18 months.

Nots

1. Keep the experimental area clean; Wear clean gloves and masks during operation; all supplies used in the experiment must be RNase free to prevent RNase.

2. All operations should be carried out on ice to prevent RNA degradation.

3. High quality RNA samples are recommended for efficient reverse transcription.

4. For your safety and health, please wear lab coats and disposable gloves for operation！

5. This product is for research use ONLY!