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0Hifair™ Ⅲ Reverse Transcriptase, Glycerol-free
Product Description
Hifair™ Ⅲ Reverse Transcriptase is an updated version of Hieff™ M-MLV (H-) Reverse Transcriptase obtained by genetic engineering technology. It has higher cDNA synthesis ability, thermal stability and reaction temperature limit (up to 60°C) than Hieff™ M-MLV (H-) Reverse Transcriptase. The synthesized cDNA product is up to 19.8 kb. Hifair™ Ⅲ Reverse Transcriptase enhances the affinity of the templates and is suitable for reverse transcription of RNA templates with complex structure or low copy genes.
Application
One-step RT-qPCR; Gene Cloning; Fluorescent Quantitation.
Unit Definition
One unit is defined as the amount of enzyme required for incorporating 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37°C using Poly(A). Oligo(dT) as template-primers.
Shipping and Storage
The product is shipped with ice packs and can be stored at 4°C for 6 months.
Cautions
1. Keep the experimental area clean and use RNase-free supplies.
2. All operations should be carried out on ice to prevent RNA degradation.
3. High quality RNA samples are recommended for efficient reverse transcription.
4. For your safety and health, please wear lab coats and disposable gloves for operation.
[1] Huang R, Xu L, Wang Y, et al. Efficient fabrication of stretching hydrogels with programmable strain gradients as cell sheet delivery vehicles. Mater Sci Eng C Mater Biol Appl. 2021;129:112415. doi:10.1016/j.msec.2021.112415(IF:7.328)
[2] Zhang ZP, Bai X, Cui WB, et al. Diterpenoid Caesalmin C Delays Aβ-Induced Paralysis Symptoms via the DAF-16 Pathway in Caenorhabditis elegans. Int J Mol Sci. 2022;23(12):6871. Published 2022 Jun 20. doi:10.3390/ijms23126871(IF:5.924)
[3] Li N, Lin SM, Li Y, Sun J, Zhang L, Chen M. An induced pluripotent stem cell line (GZHMCi004-A) derived from a fetus with heterozygous G380R mutation in FGFR3 gene causing achondroplasia. Stem Cell Res. 2021;53:102322. doi:10.1016/j.scr.2021.102322(IF:2.020)
[4] Jia N, Gong X, Chen J, et al. Generation of an induced pluripotent stem cell line (OGHFUi001-A) from a type 1 early infantile epileptic encephalopathy with ARX mutation. Stem Cell Res. 2021;53:102367. doi:10.1016/j.scr.2021.102367(IF:2.020)
[5] Chen M, Lin SM, Li N, Li Y, Li Y, Zhang L. An induced pluripotent stem cell line (GZHMCi003-A) derived from a fetus with exon 3 heterozygous deletion in RUNX2 gene causing cleidocranial dysplasia. Stem Cell Res. 2021;51:102166. doi:10.1016/j.scr.2021.102166(IF:2.020)
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