NTP Set Solution(ATP, CTP, UTP, GTP, 100 mM each)

Details:

Description

The NTP Set Solution is a convenient set of 100 mM aqueous solutions of each of ATP, CTP, UTP, GTP, supplied in separate vials. They can be used in a variety of molecular biology applications, such as in vitro transcription, RNA amplification, siRNA synthesis, etc, additionally, used as a reaction substrate or coenzyme for a variety of enzymes.
This product is a clear colorless solution prepared from trisodium salts of ATP, UTP, GTP and CTP with a purity of ≥99%, pH = 7.0±0.1 (25°C), concentration of 100 mM, and is DNase-free and RNase-free.

Feature

  • Validated, product-specific process and analytical methods
  • Product-specific stability
  • Documentation follows applicable GMP guidelines
  • AOF production process and raw materials (TSE & BSE) 
  • Nitrosamine statement
  • Regulatory support documents available
  • Large-scale production
  • Nucleotide in the multiple salt form(Na+, Tris etc) always available to meet different downstream application needs

Application

  • RNA synthesis and amplification
  • Building block for in vitro transcription

Component

Components No. Name 10133ES03
10133-A ATP Solution (100 mM) 1 mL
10133-B UTP Solution (100 mM) 1 mL
10133-C CTP Solution (100 mM) 1 mL
10133-D GTP Solution (100 mM) 1 mL

Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years.

Figures

  • Standard RNA Synthesis

Figure 1. Standard RNA was synthesized in vitro using T7 RNA synthesis kit.

The reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was analyzed by NanoDrop spectrophotometer as shown in Figure 1.

  • Capped RNA Synthesis

Figure 2. Synthesis of capped RNA in vitro.

The reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was assayed by NanoDrop spectrophotometer as shown in Figure 2A. The integrity result was analyzed by capillary electrophoresis as shown in Figure 2B.

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