Product Description

Hieff NGS™ OnePot Pro PCR-Free DNA Library Prep Kit for Illumina® is a new generation enzymatic fragmentation-based library prep kit professionally developed for Illumina® high-throughput sequencing platform. This set simplifies the operation process compared with the traditional library construction method and also greatly reduces the time and cost by performing fragmentation, end repair and dA-tailing of dsDNA in one reaction. This library prep kit has an excellent library conversion rate and is applicable for samples from all common animals, plants, microorganisms, etc., and also the FFPE samples. This upgraded kit using the latest optimized ligase greatly decreases the self-ligation rate during adapter ligation.

1、Compatible with 500 pg - 1 μg DNA samples of genomic DNA, full-length cDNA, etc.

2、High-quality fragmentation enzyme which randomly fragments dsDNA without bias

3、 Performing fragmentation, end-repair, and dA-tailing in one reaction

4、 Compatible with FFPE DNA samples

5、 Stringent quality control of batch performance and stability

Product Components

Component number	Components	12209ES24	12209ES96
12209-A	Smearase™ Buffer	240 μL	960 μL
12209-B	Smearase™ Enzyme	120 μL	480 μL
12209-C	Ligation Enhancer	720 μL	3×960 μL
12209-D	Novel T4 DNA Ligase	120 μL	480 μL

Shipping and Storage

Hieff NGS™ OnePot Pro PCR-Free DNA Library Prep Kit is shipped with ice pack and can be stored at -20℃ for 1 year.

Cautions

1. Operation

1.1 For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this product.

1.2 Please thaw the component of the kit at room temperature before use, invert and mix thoroughly, centrifuge briefly and place on ice for later use.

1.3 Please mix the reaction reagents by pipetting or vortexing gently. Vigorous vortexing may impact the library yield.

1.4 To avoid cross-contamination of samples, it is highly recommended to use filter pipette tips and change the pipette tips when drawing different samples.

1.5 It is highly recommended to pre-heat the thermocycler to the reaction temperature set for each reaction step before use. 

1.6 Improper operations likely cause aerosol pollution, affecting the accuracy of the experiment. Mandatory physical isolation of PCR reaction system preparation area and PCR product purification area is recommended. Please use special pipette tips and other equipment, and regularly clean each area (wipe with 0.5% sodium hypochlorite or 10% bleach) to ensure the cleanliness of the experimental environment.

1.7 This product is used for research ONLY!