This product is a type II restriction endonuclease derived from the recombinant protein encoded by the BspQI gene in Bacillus sphaericus expressed by E.coli. Its recognition sequence is 5'-GCTCTTCN1/N4-3'. Use to digest plasmids to prepare poly (A/T/G/C)-terminated linearized DNA fragments to obtain specific cohesive ends. This product is produced in accordance with GMP process requirements and provided in a liquid form.
|Expression Host||Recombinant E. coli with BspQI gene|
|Storage Buffer||20 mM Tris-HCl, 0.5 M KCl, 0.1 mM EDTA, 1mM DTT, 0.1% Triton X-100, 50% Glycerol|
|Unit Definition||1 unit: The amount of enzyme required to digest 1 μg of λDNA within 1 h at 50℃ in a 50 μL system|
|Components No.||Name||10664ES76 (500 U)||10664ES86 (2,500 U)||10664ES92 (10 KU)||10664ES98 (100 KU)|
|10664||BspQI GMP-grade (10 U/μL)||50 μL||250 μL||1 mL||10 mL|
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.
Figure1. The performance of YEASEN BspQI is superior.
In a 50 μL reaction system, 1 μg of λDNA was treated with the corresponding amount of BspQI (incubate at 50 °C for 60 min and then incubate at 80 °C for 20 min to inactivate BspQI). Then 20 μL of the reaction solution was loaded.
M: DNA marker
C: The control group without BspQI treatment