Description

This product is a type II restriction endonuclease derived from the recombinant protein encoded by the BspQI gene in Bacillus sphaericus expressed by E.coli. Its recognition sequence is 5'-GCTCTTCN1/N4-3'. Use to digest plasmids to prepare poly (A/T/G/C)-terminated linearized DNA fragments to obtain specific cohesive ends. This product is produced in accordance with GMP process requirements and provided in a liquid form.

Feature

• Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
• Restriction Enzyme Cut Site: GCTCTTC (1/4)
• Digestion of DNA to obtain specific sticky ends
• Isolated from a recombinant source
• Tested for the absence of endonucleases, exonucleases, RNases

Application

• Digest the plasmid to prepare a linearized DNA fragment at the end of Poly (A/T/C/G);
• Linearize plasmid template before in vitro transcription
• Restriction digest

Specification

Expression Host	Recombinant E. coli with BspQI gene
Reaction Temperature	50℃
Storage Buffer	20 mM Tris-HCl, 0.5 M KCl, 0.1 mM EDTA, 1mM DTT, 0.1% Triton X-100, 50% Glycerol
Unit Definition	1 unit: The amount of enzyme required to digest 1 μg of λDNA within 1 h at 50℃ in a 50 μL system

Components

Components No.	Name	10664ES76 (500 U)	10664ES86 (2,500 U)	10664ES92 (10 KU)	10664ES98 (100 KU)
10664	BspQI GMP-grade (10 U/μL)	50 μL	250 μL	1 mL	10 mL

Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.

Figures

Figure1. The performance of YEASEN BspQI is superior.

In a 50 μL reaction system, 1 μg of λDNA was treated with the corresponding amount of BspQI (incubate at 50 °C for 60 min and then incubate at 80 °C for 20 min to inactivate BspQI). Then 20 μL of the reaction solution was loaded.
M:  DNA marker
C: The control group without BspQI treatment