Description

UltraNuclease is a genetically engineered endonuclease from Serratia marcescens. The enzyme digests all forms of DNA/RNA moleculars into oligonucleotides of about 5 bp. The product is expressed in Escherichia coli (E. coli) and purified under the GMP environments. The nuclease is equivlent to benzonase in reducing host nucleic acid residues to pg-grade, improving the performance and safety of biological products of applications including virus purification, vaccine manufacturing, and protein/polysaccharide pharmaceutical manufacturing.  It can also used in protein study to reduce the viscosity of cell supernatant and cell lysate, increase protein purification efficiency and enhance protein functional research.

UltraNuclease is provided in the form of a sterilized reagent, eluted in buffer (20 mM Tris-HCl pH 8.0, 2 mM MgCl2, 20 mM NaCl, 50% glycerin), with the appearance of a colorless, transparent liquid. This product is produced by GMP process requirements and provided in a liquid form.

Feature

• Wide range of applications: degrade all forms of DNA and RNA 
• High purity and activity: purity ≥ 99%; specific activity ≥ 1.5×10⁶ U/mg
• Strong adaptability: strong tolerance, adapt to a variety of reaction conditions
• Comply with pharmacopeia: no animal origin; no antibiotics; lower endotoxin
• Qualification: product gets the FDA DMF filling which can shorten the customers for new drug application
• GMP-grade manufacturing: strict standards to meet the needs of large-scale use for research to manufacture

Application

• Purification of viral vaccines, viral vectors for the vaccine, and oncolytic viruses, removing DNA/RNA from proteins and other biologicals
• Reduction of viscosity caused by nucleic acids
• Sample preparation in electrophoresis and chromatography
• Prevention of cell clumping

Specification

Expression Host	Recombinant E. coli with UltraNuclease gene
Molecular Weight	26.5 kDa
Isoelectric point	6.85
Purity	≥ 99% (SDS-PAGE)
Storage Buffer	20 mM Tris-HCl pH8.0, 2 mM MgCl2, 20 mM NaCl, 50% glycerin
Unit Definition	The definition of one Activity unit (U) is the amount of enzyme used to change the absorption value of △A260 by 1.0 in 30 minutes in a 2.625 mL reaction system at 37°C with a pH of 8.0 (equivalent to complete digestion of 37 μg salmon sperm DNA into oligonucleotides).

Components

Components No.	Name	20157ES25 (25 KU)	20157ES60 (100 KU)	20157ES80 (1 MU)	20157ES90 (5 MU)
20157	UltraNuclease GMP-grade (250 U/μL)	100 μL	400 μL	4 mL	20 mL

Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years. If the product is opened and has been stored at 4℃ for more than a week, we recommend filtering the product to prevent microbial contamination.

Figures

• Quality

No.	Test Items	Competitor	Yeasen
1	Source	E. coli	E. coli
2	Activity	≥ 250 units/μL	250-300 units/μL
3	Specific Activity	≥1.1×106 U/mg	≥1.5×106 U/mg
4	Purity	≥ 99%（SDS）	≥ 99%（HPLC）
5	Endotoxin	< 0.25EU/1000U	< 0.25EU/1000U
6	Protease	None Detected	None Detected

• Reaction conditions recommend

Table 1A. The optimal reaction conditions for UCF.ME™ UltraNuclease

Factor	Optimum Condition	Effective condition
Mg2+	1-5mM	0-10mM
pH	8-9	6-10
Temperature	37℃	0-42℃
Na+/K+	0-20mM	0-150mM
PO43+	0-10mM	0-100mM
EDTA	0-0.5mM	0-5mM
Triton®X-100	0%-0.4%	0%-1%

Table 1B. The recommended enzyme usage and reaction time under the best conditions (37℃, Mg²⁺ 2mM, pH 8.0)

Amount of nuclease(Final concentration)	Reaction Time
0.25 U/mL	> 10h
2.5 U/mL	＞4h
25 U/mL	30min

• Enzyme activity analysis

Figure 1. The effect on enzyme activity of ultranuclease by temperature and pH (Figures 1A and 1B), surfactants (Figures 1C and 1D), protein denaturant (Figures 1E and 1F), cations and common chemicals (Figures 1G-1L)