UltraNuclease GMP-grade from YEASEN is expressed and purified in Escherichia coli (E. coli) by genetically engineered and prepared under the GMP environments. It can reduce the viscosity of cell supernatant and cell lysate in scientific research, increase protein purification efficiency and enhance protein functional research. The product can also reduce host nucleic acid residues to pg-grade, improving the performance and safety of biological products of applications including virus purification, vaccine manufacturing, and protein/polysaccharide pharmaceutical manufacturing. Besides, the product can also be applied to prevent the clumping of human peripheral blood mononuclear cells (PBMC) in cell therapy and vaccine development.
UltraNuclease from YEASEN is provided in the form of a sterilized reagent, eluted in buffer (20 mM Tris-HCl pH 8.0, 2 mM MgCl2, 20 mM NaCl, 50% glycerin), with the appearance of a colorless, transparent liquid. This product is produced by GMP process requirements and provided in a liquid form.
|Expression Host||Recombinant E. coli with UltraNuclease gene|
|Molecular Weight||26.5 kDa|
|Purity||≥ 99% (SDS-PAGE)|
|Storage Buffer||20 mM Tris-HCl pH8.0, 2 mM MgCl2, 20 mM NaCl, 50% glycerin|
|Unit Definition||The definition of one Activity unit (U) is the amount of enzyme used to change the absorption value of △A260 by 1.0 in 30 minutes in a 2.625 mL reaction system at 37°C with a pH of 8.0 (equivalent to complete digestion of 37 μg salmon sperm DNA into oligonucleotides).|
|Components No.||Name||20157ES25 (25 KU)||20157ES60 (100 KU)||20157ES80 (1 MU)||20157ES90 (5 MU)|
|20157||UltraNuclease GMP-grade (250 U/μL)||100 μL||400 μL||4 mL||20 mL|
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years. If the product is opened and has been stored at 4℃ for more than a week, we recommend filtering the product to prevent microbial contamination.
Figures 1. The effect of temperature and pH (Figures 1A and 1B), cations (Figures 1C and 1D) and commonly used surfactants (Figures 1E and 1F) on enzyme activity
Table 1A. The optimal reaction conditions for UCF.ME™ UltraNuclease
|Factor||Optimum Condition||Effective condition|
Table 1B. The recommended enzyme usage and reaction time under the best conditions (37℃, Mg2+ 2mM, pH 8.0)
|Amount of nuclease(Final concentration)||Reaction Time|
|0.25 U/mL||> 10h|