Ceturegel™ Matrix Phenol Red-Free,LDEV-Free

Details:

Description

Ceturegel™ Matrix LDEV-Free Matrix is a soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components are laminin, type IV collagen, heparan sulfate proteoglycan (HSPG), and nestin as well as growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator, and other growth factors contained in EHS tumors. At room temperature, it aggregates to form a biologically active three-dimensional matrix, which simulates the structure, composition, physical properties, and functions of the cell basement membrane in vivo, which is beneficial to the culture and differentiation of cells in vitro. It can be used for studies of cell morphology, biochemical function, migration, invasion, and gene expression.
The three-dimensional culture matrix formed by Matrix can promote the adherence and differentiation of epithelial cells, hepatocytes, sertoli cells, melanoma cells, vascular endothelial cells, thyroid cells, and hair follicle cells. Ceturegel™ Matrix can affect gene expression in three-dimensional cultures of murine hepatocytes and human mammary epithelial cells. At the same time, Ceturegel™ Matrix can be used as a basic scaffold for various tumor cell invasion studies
using matrigel, an essential matrix for in vitro and in vivo studies of angiogenesis, and a three-dimensional scaffold for the growth of transplanted tumor cells in in vivo animal models. Ceturegel™ Matrix also supports the regeneration of peripheral nerves and the differentiation of bovine fallopian tube epithelial cells.
YEASEN Matrix is a sterile product with a concentration of 8~20 mg/mL, which meets a variety of experimental requirements, including angiogenesis studies and tumor cell migration.

Feature

  • High Safety: No LDEV (lactate dehydrogenase elevating virus)
  • Multiple Concentration: the concentration range from 8 to 20 mg/ml
  • Batch Consistency: Strict process through the whole product life cycle including the production, quality, warehouse, etc
  • Low Endotoxin: Endotoxin content <8 EU/ml
  • Contamination Control: Adhere to the rigorous residue quality standards of mycoplasmas, bacteria, and fungi
  • High yield:>50L per single batch
  • Wide Compatibility: Compatible with almost all types of cell culture medium

Application

  • 2D Culture Support
  • Migration/Invasion
  • Biochemical Research
  • 3D & Organoid Culture
  • Angiogenesis
  • In Vivo Tumor Formation

Specification

Concentration 8-12 mg/mL
Product Type Basement Membrane Matrix
Endotoxin Level Low
Transportation Conditions Dry Ice Transportation
Product Line Ceturegel™
Form Frozen
Phenol Red Indicator None
Product specifications 5/10 mL
Species EHS Mouse Tumors
Classification Basic Type
Serum Level None
LDEV Detection None

Components

Components No. Name 40184ES08 40184ES10
40184 Ceturegel™ Matrix Phenol Red-Free,LDEV-Free 5 mL 10 mL

Storage

The product should be stored at -25℃ ~ -15℃ for 2 years.

Figures

  • Cell application validation

Figure 1A

Figure 1B

Figure 1C

Figure 1. The application validation of Ceturegel™ Matrix was as shown. The representative image of HepG2 cell stained with crystal violet after the invasion (Figure 1A). The image of the 3D culture of HepG2 cell for 4 days (Figure 1B). The representative bright field and fluorescent images of HUVEC cell angiogenesis (Figure 1C)

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