Hieff NGS™ Ultima Pro PCR Free DNA Library Prep Kit V2 is a new generation PCR Free library preparation kit specially developed and designed for the Illumina high-throughput sequencing platform. Based on the previous generation of library preparation kits, this product exhibits higher efficiencies in end repair, dA-tailing, and adapter ligation than the previous versions. The kit is compatible with most DNA sample types, including standard genomic DNA from animals/plants/microorganisms, FFPE samples, cfDNA, and ChIP DNA.
|Product Type||Library Preparation Kit|
|Input amount||5 ng - 1000 ng|
|For Use With (Equipment)||Illumina Platforms|
|Sequencing Type||Genome & DNA Sequencing|
|Product Line||DNA library Preparation|
|For Use With (Application)||NGS PCR Free library preparation|
|Quantity||8 /24/96 Reactions|
|Components No.||Name||12196ES08 (8T)||12196ES24 (24T)||12196ES96 (96T)|
|12196-A||Endprep Buffer 2.0||48 μL||144 μL||576 μL|
|12196-B||Endprep Enzyme 2.0||32 μL||96 μL||384 μL|
|12196-C||Ligation Enhancer 2.0||240 μL||720 μL||3×960 μL|
|12196-D||Rapid T4 DNA Ligase 2.0||40 μL||120 μL||480 μL|
-25℃ ~ -15℃ storage, valid for one year.
Figure 1． Comparison of the transformation efficiencies of the PCR-Free libraries
Method: Using ultrasonic interrupted calf gDNA sample as a template, 200 ng and 500 ng was used to build PCR Free library. 50 ng of each library was used for sequencing, and the library conversion efficiency was judged by the amount of data.
Results: The sequencing data volume of 12196 libraries is higher than that of competing products, and the library conversion efficiency is higher than that of competing products.
Figure 2. Comparison of GC homogeneity in PCR-Free libraries
Method: Using ultrasonic interrupted calf gDNA sample as a template, 200 ng and 500 ng was used to build PCR Free library. 50 ng of each library was used for sequencing.
Results: Compared with competing kits, 12196 sequencing data for different template inputs, the GC homogeneity was better.