N1-Me-Pseudo UTP Tris Solution GMP-grade (100 mM)

Details:

Description

N1-Me-Pseudo UTP solution is one of the most commonly used modified nucleoside triphosphates. It is mostly used as the reaction substrate or coenzyme of enzymes, such as in vitro transcription, RNA amplification, siRNA synthesis, etc. The modified mRNA containing pseudouridine has better nuclease stability and translation characteristics and changes the innate immune receptor and in vitro transcription. The interaction of RNA has a wide range of applications in the field of therapy and diagnosis.
This product is produced in accordance with GMP process requirements and provided in liquid form.

Feature

  • Validated, product-specific processes and analytical methods
  • Product-specific stability
  • Documentation follows applicable GMP guidelines
  • AOF production process and raw materials (TSE & BSE) 
  • Nitrosamine statement
  • Regulatory support documents available
  • Large-scale production
  • The increased IVT yield and the decreased dsRNA content under the optimized Tris NTP reaction system

Application

  • RNA synthesis and amplification
  • Building block for in vitro transcription

Specification

CAS No. 1428903-59-6 (free acid)
Molecular formula C10H17N2O15P3 (free acid)
Molecular weight 498.17 g/moL (free acid)
Purity ≥ 99%
Content 100mM ± 3mM
Structure

Component

Components No. Name 10657ES20 10657ES70 10657ES80 10657ES90 10657ES96 10657ES99
10657 N1-Me-Pseudo UTP Tris Solution GMP-grade (100 mM) 20 μL 100 μL 1 mL 5 mL 25 mL 500 mL

Storage

The product should be stored at -25℃ ~ -15℃ for two years.

Figures

  • Increased the IVT yield

Figure 1. The IVT yield was significantly increased under the optimized Tris NTP reaction system, compared with the sodium NTP reaction system.

  • Decreased the dsRNA content

Figure 2. The content of dsRNA was significantly decreased under the optimized Tris NTP reaction system, compared with the sodium NTP reaction system. The content of dsRNA was detected by the Dot Blot method.

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