Description

Anti-His Affinity Gel is prepared by conjugating high quality murine IgG1 monoclonal antibody with Sepharose 4B gel. This product has a high His label fusion protein loading capacity (at least 1.1 mg protein/mL gel) and less non-specific binding of miscellaneous proteins. It can be used for immunoprecipitation (IP) and purification of fusion proteins with His labels.

Features

• Specific—highly specific monoclonal antibody
• High yield and high purity
• Versatile—It can be used for protein purification or COIP
• Convenient—reagents and peptides to elute are available separately

Applications

• Immunoprecipitation (IP)
• Purification of fusion proteins with His labels.

Specifications

Isotype	Mouse IgG1
Purity	Protein A
Antibody Concentration	7.5 g antibody/L Gel
Protein binding capacity	≥1.1 mg protein/mL Gel
Storage buffer	TBS, 50% glycerin, pH7.4, contains 0.02% (w/v) sodium azide

Components

Components No.	Name	20589ES03	20589ES08	20589ES25	20589ES60
20589	Anti-His Affinity Gel	1 mL	5 mL	25 mL	100 mL

Storage

The unopened products should be stably stored at -25℃ ~ -15℃ for 1 year. Do not freeze in solution without glycerin!