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High-quality isothermal amplification raw materials ——Making RT -LAMP more sensitive and faster!

Isothermal amplification technology can achieve the purpose of amplifying specific nucleic acid sequences under constant temperature conditions. Due to the characteristics of no special equipment, short amplification time, and high sensitivity, it has shown good application prospects in on-site detection and point-of-care diagnosis and is very suitable for the development of molecular diagnostic tools.

In the context of the novel coronavirus pandemic with increasing strains and infectivity, Isothermal amplification technology can detect the novel coronavirus rapidly with simple instruments, helping to solve the problem that some COVID-19 patients can't be quickly confirmed due to the long detection time and high requirements for detection equipment and reagents of traditional PCR techniques.

 

[New upgrade] Yeasen Hieff™ Bst Plus DNA Polymerase

[New Arrival] Yeasen RT-LAMP pH Sensitive Dyestuff Kit

Product Selection Guide

 

[New upgrade] Yeasen Hieff™ Bst Plus DNA Polymerase

The newly upgraded Hieff™ Bst Plus DNA Polymerase (Cat#14402ES, 14403ES) is obtained by expressing and purifying the DNA polymerase gene of Bacillus stearothermophilus sp lacking the 5′→3′ exonuclease domain in E.coli. The enzyme strand displacement ability is strong, and has the advantages of high sensitivity, high amplification efficiency, and high dUTP tolerance, and can be widely used in the real-time detection of pathogens based on isothermal amplification technology.

Fast and high sensitivity

Yeasen Hieff™ Bst Plus DNA Polymerase has high sensitivity and can detect the target gene as low as 5 copies. The amount of template is at the femtogram (fg) level, and the amplification rate is faster than competing products.

Figure 1. RT-LAMP reaction was carried out with Yeasen Hieff™ Bst Plus DNA Polymerase (Orange) and Bst enzymes of competitor N (Gray) and competitor T (Blue) to amplify SARS-CoV-2. The results show that Yeasen Hieff™ Bst Plus DNA Polymerase can always reach the threshold faster than competing products, and the amplification rate is faster.

[New Arrival] Yeasen RT-LAMP pH Sensitive Dyestuff Kit

Based on the newly upgraded Hieff™ Bst Plus DNA Polymerase with high sensitivity, amplification efficiency and dUTP tolerance, Yeasen has launched the RT-LAMP pH Sensitive Dyestuff Kit (Cat#13906ES).

Product Features

1. Provides a convenient one-step solution for LAMP and RT-LAMP.

2. Complete rapid detection in 10 minutes, and the sensitivity can reach 50 copies/Rxn.

3. The test results are visible to the naked eye, and the reagents can be lyophilized.

4. The premix contains the Heat-Liable UDG enzyme, which can remove the aerosol contamination of amplification products containing dU.

5. Add Murine RNase inhibitor to prevents RNA sample degradation and without inhibition of amplification.

Performance display


Figure 2. The amplification results of RT-LAMP pH Sensitive Dyestuff Kit (Cat#13906ES) can be judged by the naked eye. The reaction time is 30 min. The positive reaction wells are orange, and the negative reaction wells are magenta. (Note: Freeze-dried samples are shown in the image).

Product Selection Guide

 

Product Positioning

Product name

Cat#

Highly sensitive Bst enzyme

Hieff™ Bst Plus DNA Polymerase (40 U/μL)

14402ES

Hieff™ Bst Plus DNA Polymerase (2000 U/μL)

14403ES

Dye Method RT-Lamp Display Kit

RT-LAMP Dye Assay Kit (UDG plus)

13762ES

pH Sensitive Dye Method RT-Lamp Display Kit (Lyophilized)

RT-LAMP pH Sensitive Dyestuff Kit

13906ES

Reverse transcriptase suitable for RT-Lamp

Hifair™ Ⅲ Reverse Transcriptase

11111ES

Hifair™ Ⅲ Reverse Transcriptase, Glycerol-free

11297ES

Murine RNase Inhibitor

Murine RNase inhibitor (40 U/μL)

10603ES

Murine RNase inhibitor (200 U/μL, Glycerol-free)

10703ES

Heat-labile UDG

Uracil DNA Glycosylase (UDG/UNG), heat-labile, 1 U/μL

10303ES

High-purity dUTP

dUTP (100 mM Solution)

10128ES

Regarding reading:

[New Arrival] Hieff™ Bst Plus DNA Polymerase-Isothermal Amplification Core Enzyme!