Proteinase K (20 mg/mL)

Product Description

Proteinase K is a serine protease with wide cleavage activity, which can cleave the carboxyl terminal peptide bond of aliphatic and aromatic amino acids. Its relative molecular weight is about 29.3 kDa. Proteinase K is widely used in the preparation of chromosomal DNA for pulse electrophoresis, Western blotting and the removal of nuclease from DNA and RNA preparation. Denaturant such as SDS (1%) can improve its activity. In addition, the common working concentration is 50-100 μg/mL and the specific working concentration according to whether the buffer contains SDS, urea, pH, temperature and other factors.

Product Properties

Source	Recombinant Yeast
Appearance	Sterile solution
Specific Activity	≥30 U/mg
Unit Definition	One unit is defined as the amount of proteinase K that will liberate 1μmol tyrosine per minute at 37℃ @ pH 7.5.
Purification	Ni column affinity purification
Reaction Condition	The reaction buffer should be incubated at 37℃-70℃ for 1h, and the recommended reaction temperature is 70℃.
Inactivation method	The reaction system can be inactivated by adding PMSF or DFP inhibitor, and partially inactivated by incubation at 65℃ for 10-15 min.
Activity Range	It has activity in a wide range of pH (4.0-12.0) and the optimal pH range is 7.5-9.0.
RNase and DNase	None

Shipping and Storage

The product is shipped at room temperature and can be stored at 4°C for 1 years, stored at -20°C for a longer time. Avoid repeated freeze thawing.

Cautions

1. If the product needs to be used repeatedly, it should be filtered, sterilized and stored separately.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. This product is for research use ONLY!