RT-LAMP Dye Assay Kit (UDG plus)



RT-LAMP Dye Assay Kit (UDG plus) contains fluorescent dye, Hieff™ Bst Plus DNA Polymerase, heat-resistant Hifair™ Ⅲ Reverse Transcriptase, MRI, and UDGas applied to LAMP reaction with RNA as template. This product is with high sensitivity and high specificity.


  • High Sensitivity:50 copies/T, 25 μL system;
  • High stability: stable at 37°C for 5 days with good stability, and can withstand repeated freezing and thawing more than 10 times;
  • Specificity: 48-well NTC has no peaks at all, and the specificity is strong;


  • Rapid detection of pathogenic bacteria
  • Rapid detection of pet pathogens
  • On-site detection of animal husbandry


Detection method Fluorescent dye method
PCR method RT-LAMP
Polymerase Hieff™ Bst Plus DNA Polymerase
Type of sample RNA
LOD 50 copies/T
Specificity 100%


Components No. Name 13762ES60 (100 T) 13762ES80 (1000 T)
13762-A Bst Buffer 600 µL 6 mL
13762-B Bst Enzyme Mix(UDG plus) 100 µL 1 mL


Part 1: 13762-A should be protected from light and can be stored at -20°C for 6 months.
Part 2: 13762-B can be stored at -20°C for 6 months.


  • High Sensitivity

Figure 1: Sensitivity Test

Red: 50 copies/T, response less than 15 minutes
Figure 1: The copy numbers of 50 copies/Tweres tested. The test results showed that within 15 minutes, the results of 50 copies/T performed better, and the detection rate reached 100%.


  • Stability

Figure 2.Stability testing at the same temperature

Red: 37℃ 0 day  Blue: 37℃ 1 day  Green: 37℃ 3 days  Purple: 37℃ 5 days
0 days at 37°C as a control and stability tests were carried out for 1, 3, and 5 days at the same temperature, indicating that it can still maintain a relatively stable effect at 37°C for 5 days.


Figure 3. -20℃ repeated freeze-thaw stability test

Red: Repeat freeze-thaw 0 times
Blue: Repeat freeze-thaw 5 times
Green: Repeat freeze-thaw 10 times


  • Specificity

Figure 4. 48-well NTC-specific assay

48-well NTC detection was used in the specific detection of the product, and no peak was detected, indicating that the specificity was better.

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