dsDNA HS assay kit



dsDNA HS Assay Kit is a fast, sensitive and accurate double-stranded DNA (dsDNA) fluorescence quantitative detection kit. The kits include concentrated assay reagents, dilution buffer, and DNA standards. This kit is highly selective for dsDNA over RNA and is accurate for initial sample concentration from 10 pg/μL to 100 ng/μL. It is an ideal kit for DNA sample quantification such as NGS input DNA quantification and DNA library quantification. The kit has good tolerance to conventional contaminants such as salts, free nucleotides, solvents, detergents, and proteins.


  • High sensitive: the concentrations ranging from 10 pg/µ l to 100 ng/µL of dsDNA can be accurately quantified;
  • High specificity: this product is highly selective to dsDNA and is not affected by RNA, has good tolerance to some conventional pollutants, such as salt, free nucleotide, protein, solvent, detergent, etc;
  • Easy to use: the dsDNA sample is to be tested and test it with a Qubit fluorometer or Fluorescent microplate reader at room temperature


  • dsDNA quantification with Qubit or Fluorescent microplate reader
  • NGS library quantification


Assay dsDNA Quantitation
Excitation/Emission 480/520
For Use With (Equipment) Qubit Fluorometer, Microplate Reader
No. of Reactions 100T/500T
Product Line Qubit Quantitation
Quantitation Range 10 pg/μL to 100 ng/μL
Sample Volume 1 μL to 20 μL
Detection Method Fluorescent


Components No. Name Concentration 12640ES60 (100T) 12640ES76 (500T)
12640-A dsDNA Reagent 200×concentrate in DMSO 250 μL 1.25 mL
12640-B dsDNA Buffer Not applicable 50 mL 250 mL
12640-C dsDNA Standard 1 0 ng/μL in TE buffer 1 mL 5×1 mL
12640-D dsDNA Standard 2 10 ng/μL in TE buffer 1 mL 5×1 mL

Shipping and Storage

All the components are shipped with an ice pack and can be stored at 2-8℃ away from light for one year.


Product stability test. Super stability, high relevance of test results within the validity period

Citations & References:

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[3] An C, Liu W, Zhang Y, et al. Continuous microfluidic encapsulation of single mesenchymal stem cells using alginate microgels as injectable fillers for bone regeneration. Acta Biomater. 2020;111:181-196. doi:10.1016/j.actbio.2020.05.024(IF:7.242)

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[6] Zhao H, Tang X, Wu M, et al. Transcriptome Characterization of Short Distance Transport Stress in Beef Cattle Blood. Front Genet. 2021;12:616388. Published 2021 Feb 10. doi:10.3389/fgene.2021.616388(IF:4.599)

[7] Gao ZR, Liu Q, Zhao J, et al. A comprehensive analysis of the circRNA-miRNA-mRNA network in osteocyte-like cell associated with Mycobacterium leprae infection. PLoS Negl Trop Dis. 2022;16(5):e0010379. Published 2022 May 2. doi:10.1371/journal.pntd.0010379(IF:4.411)

[8] Li J, Li X, Li M, et al. Differential early diagnosis of benign versus malignant lung cancer using systematic pathway flux analysis of peripheral blood leukocytes. Sci Rep. 2022;12(1):5070. Published 2022 Mar 24. doi:10.1038/s41598-022-08890-x(IF:4.380)

[9] Liang H, Zhang X, Ma Z, et al. Association of CYP3A5 Gene Polymorphisms and Amlodipine-Induced Peripheral Edema in Chinese Han Patients with Essential Hypertension. Pharmgenomics Pers Med. 2021;14:189-197. Published 2021 Feb 2. doi:10.2147/PGPM.S291277(IF:3.912)

[10] Bing XL, Zhao DS, Peng CW, Huang HJ, Hong XY. Similarities and spatial variations of bacterial and fungal communities in field rice planthopper (Hemiptera: Delphacidae) populations. Insect Sci. 2020;27(5):947-963. doi:10.1111/1744-7917.12782(IF:2.791)

[11] Zhang Y, Dai J, Yan L, Sun Y. Intra-articular injection of decellularized extracellular matrices in the treatment of osteoarthritis in rabbits. PeerJ. 2020;8:e8972. Published 2020 Apr 22. doi:10.7717/peerj.8972(IF:2.379)

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