Clodronate Liposomes(From Vrije Universiteit Amsterdam)


Product Description

Clodronate liposomes are applicable to a variety of injection methods, such as intravenous injection, intraperitoneal injection, subcutaneous injection, intranasal injection and testicular injection. The amount of injection was related to the weight of mice, injection cycle, injection method, and experimental purpose.

Product characteristics 


10 mM Na2HPO4,10 mM NaH2PO4,140 mM NaCl


5 mg/mL

Lipid composition

Phosphatidylcholine and cholesterol


Shipping and Storage

The components are shipped with ice pack and can be stored at 4 ℃ for 3 months. Cannot be frozen!


1. Before use, it must be fully mixed and restored to room temperature.

2. For research use only! 


Please refer to relevant literature for specific injection volume, and explore and optimize according to your own experimental conditions (such as experimental purpose, injection method, injection cycle).

Intraperitoneal injection

1. Before injection, remove clodronate liposomes and sterile PBS (for injection) from the refrigerator. Naturally return to room temperature (18 ℃).

[Notes]: Clodronate liposomes suspension shall not be frozen and shall not exceed  30 ℃.

2. Upside down for 8-10 times. Connect a 26 gauge needle to a 1ml syringe and suck 200 μL Clodronate liposomes .

3. Grab enough skin behind the ears of the mouse and tail with the left hand to fix the head and limbs.

4. Tilt the mouse slightly and let the head face the ground, so that the organ originally concentrated at the lower right of the abdomen moves towards the head and away from the injection site.

5. Before injection, reverse the syringe for 6 times and mix clodronate liposomes.

[Notes]: Long time placement will cause liposomes to precipitate in the syringe, resulting in uneven concentration during the injection.

6. The needle is inserted into the lower right side of the abdomen at an angle of 30 degrees. 200 μL injections respectively clodronate liposomes (experimental group) and PBS (control group).



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