Polyacrylamide gel (PAGE gel) is often used for protein electrophoresis to realize protein separation. This kind of gel is generally composed of concentrated gel and separation gel. The former plays the role of concentrating protein samples, while the latter separates proteins of different sizes according to the concentration of acrylamide monomer and N,N-methylenebisacrylamide (methyleneacrylamide) crosslinking agent used in the gel.
This kit provides a variety of reagents required for quick preparation of PAGE gel. Users only need to prepare their own gel preparation equipment to prepare gel, which greatly simplifies the gel preparation process. The gel prepared by this kit can only be used for denaturing PAGE gel electrophoresis. This specification can prepare about 125 pieces of mini gel (calculated by 0.75 mm thick gel).
|SDS-PAGE concentrated gel concentration||4.2 %|
|SDS-PAGE separation gel concentration||12.5 %|
|Separation range (kDa)||15-60|
|Components No.||Name||20326ES62 (125 mini gels)|
|20326-A||12.5 % - separation gel buffer||250 mL|
|20326-B||12.5 % - separation gel solution||250 mL|
|20326-C||12.5 % - color concentrated gel buffer||80 mL|
|20326-D||12.5 % - concentrated gel solution||80 mL|
|20326-E||Prepare gel cup||3|
|20326-F||Improved ammonium persulfate solution||8 mL|
The product should be stored at 2℃~8℃. Among them, 20326-F (Improved ammonium persulfate solution) can be stored at -25℃~-15℃ for 12 months if it is not used for a long time.
Figure 1. The PAGE gel preparation effect with clear Marker band