Description

BL21 (DE3) Chemically Competent Cell from YEASEN is a chemically transformed competent cell prepared on the basis of the Escherichia coli BL21 (DE3) strain, and is a high-efficiency protein expression host with T7 RNA polymerase as the expression system. It can simultaneously express T7 RNA polymerase and E. coli RNA polymerase for protein expression of pET series, pGEX, pMAL and other plasmids. The product is Suitable for recombinant expression of non-viral proteins. The conversion efficiency of more than 10⁷ cfu/μg DNA was detected using pUC19.

BL21 (DE3) competent cell genotypes: F ^-ompT hsdS_B (r_B^-m_B^-) gal dcm (DE3).

Features

•High transformation efficiency: >1×10⁷ cfu/μg pUC19 DNA

•Specifically constructed for high-level expression of recombinant proteins

Applications

BL21 (DE3) Competent Cells are suitable for high-level T7 expression of recombinant proteins.

Specifications

Species	Escherichia coil
Cell type	Chemical competent cell
Whether methylated DNA can be cloned	Yes
Efficiency	＞1×107
Blue-white spot screening	No
Bacterial or yeast strains	BL21 (DE3)
Amplification of the ccdB-containing vector	It is not suitable for amplification of ccdB-containing vectors

Components

Components No.	Name	11804ES80
11804	BL21 (DE3) Chemically Competent Cell	10×100 μL

 Storage

The product should be stored at -85~-65℃ for six months. Do not store the product in -25~-15℃ or liquid nitrogen.

Ver.EN20240118