UCF.ME® High Affinity RNase Inhibitor (40 U/μL)

Product description

UCF.ME^TM High Affinity RNase Inhibitor (40 U/μL) is a recombinant porcine RNase inhibitor expressed in E. coli. It specifically inhibits the activity of RNases A, B and C through binding noncovalently. This product can be compatible with Hifair^TM Ultra Reverse Transcriptase (200 U/μL) (Cat#14604ES) and various DNA polymerases verified by RT-PCR and RT-qPCR. UCF.ME^TM High Affinity RNase Inhibitor (40 U/μL) is lower with the purification process specially developed by YEASEN, which is suitable for application with more stringent requirements on background bacteria, such as pathogen microorganism detection.

Specifications

Cat.No.	14675ES05 / 14675ES20 / 14675ES60
Size	2 KU / 20 KU / 100 KU
Unit Definition	The required amount of RNase Inhibitor to inhibit 50% activity of 5-ng RNase A is defined as one unit. The activity of RNase A is measured by hydrolyzing of cyclic 2', 3'-CMP to generate 3'-CMP.

Components

Name	14675ES05	14675ES20	14675ES60
UCF.METM High Affinity RNase Inhibitor (40 U/μL)	50 μL	500 μL	2.5 mL

Storage

This product should be stored at -25~-15℃ for 2 years.

Instructions

1. Add the following components to a nuclease-free microcentrifuge tube:

Components	Volume (μL)
RNase-free ddH2O	to 20
5×HifairTM Ultra Reverse Transcriptase Reaction Buffer*	4
Oligo (dT)18 (50 μM)	1
dNTP Mix (10 mM each)	1
UCF.METM High Affinity RNase Inhibitor (40 U/μL)	0.5-1
HifairTM Ultra Reverse Transcriptase (200 U/μL)*	1
RNA template	Total RNA:1 ng-5 μg

*According to the specific experimental application, 14604ES can be purchased for use together.

2.Thermal cycling protocol

Temperature	Time
50℃*	15 min
85℃**	5 min

*Reverse transcription temperature: When used with 14604ES, 50℃ is recommended; For high GC content templates or complex templates, the reverse transcription temperature can be increased to 55℃-60℃.

**inactivate the reaction by heating at 85℃ for 5 min.

3. The products of reverse transcriptase reaction can be immediately used for subsequent PCR or qPCR reactions, and can be stored at -25~-15℃ for short-term storage. If long-term storage is required, it is recommended to store them at -80℃ after packaging to avoid repeated freeze-thaw products.

 Notes

1. This product is for scientific research purposes only.

2. Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety.

Ver. EN20230921