MycAway -Color One-Step Mycoplasma Detection Kit UNG Plus



The MycAwayTM Plus-Color One-Step Mycoplasma Detection Kit is a rapid detection product for mycoplasma contamination in cell culture which was developed based on the unique isothermal amplification technology of YEASEN. Compared with the previous mycoplasma detection products, this kit is optimized and has greatly reduced the false positive rate, improved the accuracy of the test, and enhanced the identification of negative and positive results. At the end of amplification, the sample can be left at room temperature for a period of time without the change from negative to positive, and thus will not affect the judgment of the results.

The principle is that if the cell culture is contaminated with mycoplasma, the conserved sequences of mycoplasma DNA will be amplified heavily and rapidly, causing the color of the reaction solution to change from blue-purple to sky-blue, and the result is visible to the naked eye, without electrophoresis.

MycAwayTM Plus-Color One-Step Mycoplasma Detection Kit can detect multiple mycoplasma species, including eight that are common in cell culture. The traditional nested PCR method for mycoplasma detection is susceptible to influence by the inhibitors in cell culture supernatant and showed a false-negative result. Also if needed to perform the electrophoresis, cap opening may increase the contamination and lead to a false-positive result. The one-step mycoplasma detection kit has none of these disadvantages, also its detection sensitivity and accuracy are much higher than those of the PCR method.


  • The one-step operation results in less than 60 minutes
  • Use a water bath or PCR instrument to test
  • 27 mycoplasma species were accurately detected by the kit, including 8 common species
  • Results just identified by the visual inspection


  • Mycoplasma Detection


Components No. Name 40612ES25 (25 T) 40612ES60 (100 T)
40612-A MycAway™ -Color Lamp 425 μL 4 × 425 μL
40612-B MycAway™-Color Lamp Primer 50 μL 4 × 50 μL
40612-C Positive Control 10 μL 4 × 10 μL
40612-D Mineral oil 500 μL 4 × 500 μL

Shipping and Storage

All the components are shipped with dry ice and can be stored at -15℃ ~ -25℃ for 18 months. Please keep away from the light.


  • The detection procedure

Figure 2. The detection procedure by MycAway™ Plus–Color One-Step Mycoplasma Detection Kit from YEASEN

  • Display of detection result

Figure 2. The detection result by MycAway™ Plus–Color One-Step Mycoplasma Detection Kit from YEASEN

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