Hifair™ Ⅲ 1st Strand cDNA Synthesis SuperMix for qPCR (gDNA Digester Plus)

Details:

Description

Hifair™ Ⅲ 1st Strand cDNA Synthesis SuperMix for qPCR (gDNA Digester Plus) is a readjust premix developed based on Hifair™ⅢReverse Transcriptase. Compared with Hifair™ ⅡReverse Transcriptase, Hifair™ Ⅲ Reverse Transcriptase has significantly higher thermal stability and can withstand reaction temperatures up to 60℃, and is suitable for Reverse transcription of RNA templates with complex secondary structures. At the same time, the enzyme enhances the affinity with templates, which is ideal for the reverse transcription of a small number of templates and low-copy genes.

The premix contains 5×gDNA Digester Mix and 4×Hifair™ Ⅲ SuperMix Plus. 5×gDNA Digester Mix can remove residual genomic DNA contamination in RNA templates to ensure more reliable follow-up results. 4×Hifair™ Ⅲ SuperMix Plus contains all components required for Reverse Transcriptase (Buffer, dNTP, Hifair™ Ⅲ Reverse Transcriptase, RNase inhibitor, Random Primers/Oligo (dT)18 Primer Mix), RNA template and Rnase-free H2O are added to reverse transcription, and gDNA digester is terminated simultaneously to ensure the integrity of cDNA.

The product is suitable for two-step RT-QPCR detection, specially optimized for qPCR, proportionally optimized Random Primers/Oligo (dT)18 Primer Mix, enabling cDNA synthesis to be initiated from all regions of RNA transcript with the same reverse transcription efficiency. The authenticity and repeatability of qPCR results are guaranteed to the greatest extent. The reverse transcription product is compatible with SYBR Green method and TaqMan probe method qPCR, Hifair™ UNICON™ qPCR SYBR Green Master Mix, Hifair™ qPCR SYBR Green Master Mix or Hifair™ qPCR TaqMan Probe Master Mix can be selected according to experimental purposes, is used in combination to perform high-performance gene expression analysis.

Features

  • High-performance reverse transcription
  • Increase reaction temperature - First strand cDNA can be synthesized in the temperature range of 55 to 60°C
  • Efficient removal of genomic residues
  • Broad linear detection range

Applications

  • Construction of cDNA library
  • Antisense RNA synthesis

Specifications

Type of end product cDNA (First-Strand)
PCR method RT-PCR
Type of reagent Reverse Transcription
Type of sample RNA
Optimal reaction temperature 55℃-60℃
Reverse transcriptase M-MLV

Components

Components No. Name 11141ES10 (10T) 11141ES60 (100T)
11141-A RNase-free H2O 1 mL 2×1 mL
11141-B 5×gDNA digester Mix 30 μL 300 μL
11141-C 4×HifairTM Ⅲ SuperMix plus 50 μL 500 μL

Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 18 months.

Figures

Figure 1. Excellent reverse transcription efficiency, suitable for genes with different expression abundances with different GC contents

Figure 2. Linear assay range Total RNA 10 pg~5 μg

Figure 3. Efficient removal of genomic residues

Cited from"An RNA-cleaving threose nucleic acid enzyme capable of single point mutation discrimination. Nat Chem . 2022 Mar;14(3):350-359. doi: 10.1038/s41557-021-00847-3."

Cited from"The tetratricopeptide repeat protein OsTPR075 promotes heading by regulating florigen transport in rice. Plant Cell . 2022 Sep 27;34(10):3632-3646. doi: 10.1093/plcell/koac190."

Citations & References:

[1] Song JM, Xie WZ, Wang S, et al. Two gap-free reference genomes and a global view of the centromere architecture in rice. Mol Plant. 2021;14(10):1757-1767. doi:10.1016/j.molp.2021.06.018(IF:13.164)

[2] Chen Y, Shen J, Zhang L, et al. Nuclear translocation of OsMFT1 that is impeded by OsFTIP1 promotes drought tolerance in rice. Mol Plant. 2021;14(8):1297-1311. doi:10.1016/j.molp.2021.05.001(IF:13.164)

[3] Yang L, Chen Y, Xu L, et al. The OsFTIP6-OsHB22-OsMYBR57 module regulates drought response in rice. Mol Plant. 2022;15(7):1227-1242. doi:10.1016/j.molp.2022.06.003(IF:13.164)

[4] Zhang L, Zhang F, Zhou X, et al. The tetratricopeptide repeat protein OsTPR075 promotes heading by regulating florigen transport in rice [published online ahead of print, 2022 Jun 28]. Plant Cell. 2022;koac190. doi:10.1093/plcell/koac190(IF:11.277)

[5] Mao Z, Xiao H, Shen P, et al. KRAS(G12D) can be targeted by potent inhibitors via formation of salt bridge. Cell Discov. 2022;8(1):5. Published 2022 Jan 25. doi:10.1038/s41421-021-00368-w(IF:10.849)

[6] Jiang R, Su G, Chen X, et al. Esculetin inhibits endometrial cancer proliferation and promotes apoptosis via hnRNPA1 to downregulate BCLXL and XIAP [published online ahead of print, 2021 Sep 1]. Cancer Lett. 2021;521:308-321. doi:10.1016/j.canlet.2021.08.039(IF:8.679)

[7] Ou-Yang K, Feng T, Han Y, Li G, Li J, Ma H. Bioaccumulation, metabolism and endocrine-reproductive effects of metolachlor and its S-enantiomer in adult zebrafish (Danio rerio). Sci Total Environ. 2022;802:149826. doi:10.1016/j.scitotenv.2021.149826(IF:7.963)

[8] Xie Y, Hu X, Li S, et al. Pharmacological targeting macrophage phenotype via gut-kidney axis ameliorates renal fibrosis in mice. Pharmacol Res. 2022;178:106161. doi:10.1016/j.phrs.2022.106161(IF:7.658)

[9] Wang T, Yang J, Lin G, et al. Effects of Dietary Mannan Oligosaccharides on Non-Specific Immunity, Intestinal Health, and Antibiotic Resistance Genes in Pacific White Shrimp Litopenaeus vannamei. Front Immunol. 2021;12:772570. Published 2021 Nov 24. doi:10.3389/fimmu.2021.772570(IF:7.561)

[10] Tie HM, Sun RX, Yu DW, et al. The apoptosis of grass carp (Ctenopharyngodon idella) muscle during postmortem condition regulated by the cytokines via TOR and NF-κB signaling pathways. Food Chem. 2022;369:130911. doi:10.1016/j.foodchem.2021.130911(IF:7.514)

[11] Chai X, Sun H, Zhou W, et al. Discovery of N-(4-(Benzyloxy)-phenyl)-sulfonamide Derivatives as Novel Antagonists of the Human Androgen Receptor Targeting the Activation Function 2. J Med Chem. 2022;65(3):2507-2521. doi:10.1021/acs.jmedchem.1c01938(IF:7.446)

[12] Yang G, Chen BX, Chen T, et al. BYPASS1-LIKE regulates lateral root initiation via exocytic vesicular trafficking-mediated PIN recycling in Arabidopsis. J Integr Plant Biol. 2022;64(5):965-978. doi:10.1111/jipb.13243(IF:7.061)

[13] Feng YJ, You XJ, Ding JH, Zhang YF, Yuan BF, Feng YQ. Identification of Inosine and 2'-O-Methylinosine Modifications in Yeast Messenger RNA by Liquid Chromatography-Tandem Mass Spectrometry Analysis. Anal Chem. 2022;94(11):4747-4755. doi:10.1021/acs.analchem.1c05292(IF:6.986)

[14] Shu C, Wang L, Zou C, et al. Function of Foxl2 and Dmrt1 proteins during gonadal differentiation in the olive flounder Paralichthys olivaceus [published online ahead of print, 2022 Jun 16]. Int J Biol Macromol. 2022;215:141-154. doi:10.1016/j.ijbiomac.2022.06.098(IF:6.953)

[15] Yang S, Liu P, Gao T, et al. Every road leads to Rome: therapeutic effect and mechanism of the extracellular vesicles of human embryonic stem cell-derived immune and matrix regulatory cells administered to mouse models of pulmonary fibrosis through different routes. Stem Cell Res Ther. 2022;13(1):163. Published 2022 Apr 12. doi:10.1186/s13287-022-02839-7(IF:6.832)

[16] Cai Z, Yang C, Liao J, Song H, Zhang S. Sex-biased genes and metabolites explain morphologically sexual dimorphism and reproductive costs in Salix paraplesia catkins. Hortic Res. 2021;8(1):125. Published 2021 Jun 1. doi:10.1038/s41438-021-00566-3(IF:6.793)

[17] Li YY, Cen H, Gong BN, et al. TCR-Induced Tyrosine Phosphorylation at Tyr270 of SUMO Protease SENP1 by Lck Modulates SENP1 Enzyme Activity and Specificity. Front Cell Dev Biol. 2022;9:789348. Published 2022 Feb 2. doi:10.3389/fcell.2021.789348(IF:6.684)

[18] Yang R, Chen X, Huang Q, et al. Mining RNA-Seq Data to Depict How Penicillium digitatum Shapes Its Transcriptome in Response to Nanoemulsion. Front Nutr. 2021;8:724419. Published 2021 Sep 14. doi:10.3389/fnut.2021.724419(IF:6.576)

[19] Cheng GP, Guo SM, Yin Y, Li YY, He X, Zhou LQ. Aberrant Expression of Mitochondrial SAM Transporter SLC25A26 Impairs Oocyte Maturation and Early Development in Mice. Oxid Med Cell Longev. 2022;2022:1681623. Published 2022 Apr 13. doi:10.1155/2022/1681623(IF:6.543)

[20] Yang L, Li Z, Chen Y, et al. Elucidating the Novel Mechanism of Ligustrazine in Preventing Postoperative Peritoneal Adhesion Formation. Oxid Med Cell Longev. 2022;2022:9226022. Published 2022 Mar 10. doi:10.1155/2022/9226022(IF:6.543)

[21] Bai J, Yin L, Yu WJ, Zhang YL, Lin QY, Li HH. Angiotensin II Induces Cardiac Edema and Hypertrophic Remodeling through Lymphatic-Dependent Mechanisms. Oxid Med Cell Longev. 2022;2022:5044046. Published 2022 Feb 18. doi:10.1155/2022/5044046(IF:6.543)

[22] Wu Y, Zhang X, Feng H, et al. Exploration of Redox-Related Molecular Patterns and the Redox Score for Prostate Cancer. Oxid Med Cell Longev. 2021;2021:4548594. Published 2021 Nov 11. doi:10.1155/2021/4548594(IF:6.543)

[23] Wei YY, Zhang DW, Ye JJ, et al. Interleukin-6 neutralizing antibody attenuates the hypersecretion of airway mucus via inducing the nuclear translocation of Nrf2 in chronic obstructive pulmonary disease. Biomed Pharmacother. 2022;152:113244. doi:10.1016/j.biopha.2022.113244(IF:6.530)

[24] Li H, Liu S, Chen L, Luo J, Zeng D, Li X. Juvenile hormone and transcriptional changes in honey bee worker larvae when exposed to sublethal concentrations of thiamethoxam. Ecotoxicol Environ Saf. 2021;225:112744. doi:10.1016/j.ecoenv.2021.112744(IF:6.291)

[25] Luo Y, Tao T, Tao R, Huang G, Wu S. Single-Cell Transcriptome Comparison of Bladder Cancer Reveals Its Ecosystem. Front Oncol. 2022;12:818147. Published 2022 Feb 21. doi:10.3389/fonc.2022.818147(IF:6.244)

[26] Peng L, Peng JY, Cai DK, et al. Immune Infiltration and Clinical Outcome of Super-Enhancer-Associated lncRNAs in Stomach Adenocarcinoma. Front Oncol. 2022;12:780493. Published 2022 Mar 3. doi:10.3389/fonc.2022.780493(IF:6.244)

[27] Zhang Y, Ma Y, Liu R, Li G. Genome-Wide Characterization and Expression Analysis of KH Family Genes Response to ABA and SA in Arabidopsis thaliana. Int J Mol Sci. 2022;23(1):511. Published 2022 Jan 3. doi:10.3390/ijms23010511(IF:5.924)

[28] Zhenzhen L, Wenting L, Jianmin Z, et al. miR-146a-5p/TXNIP axis attenuates intestinal ischemia-reperfusion injury by inhibiting autophagy via the PRKAA/mTOR signaling pathway. Biochem Pharmacol. 2022;197:114839. doi:10.1016/j.bcp.2021.114839(IF:5.858)

[29] Zhao Z, Yang S, Deng Y, et al. Naringin Interferes Doxorubicin-Induced Myocardial Injury by Promoting the Expression of ECHS1. Front Pharmacol. 2022;13:859755. Published 2022 Apr 12. doi:10.3389/fphar.2022.859755(IF:5.811)

[30] Wu S, Lin C, Zhang T, et al. Pentamidine Alleviates Inflammation and Lipopolysaccharide-Induced Sepsis by Inhibiting TLR4 Activation via Targeting MD2. Front Pharmacol. 2022;13:835081. Published 2022 Feb 23. doi:10.3389/fphar.2022.835081(IF:5.811)

[31] Huang Q, Lin J, Huang S, Shen J. Impact of Qi-Invigorating Traditional Chinese Medicines on Diffuse Large B Cell Lymphoma Based on Network Pharmacology and Experimental Validation. Front Pharmacol. 2021;12:787816. Published 2021 Dec 9. doi:10.3389/fphar.2021.787816(IF:5.811)

[32] Wu W, Zhang X, Zhou J, et al. Clemastine Ameliorates Perioperative Neurocognitive Disorder in Aged Mice Caused by Anesthesia and Surgery. Front Pharmacol. 2021;12:738590. Published 2021 Aug 23. doi:10.3389/fphar.2021.738590(IF:5.811)

[33] Huang Q, Lin J, Huang S, Shen J. Impact of Qi-Invigorating Traditional Chinese Medicines on Diffuse Large B Cell Lymphoma Based on Network Pharmacology and Experimental Validation. Front Pharmacol. 2021;12:787816. Published 2021 Dec 9. doi:10.3389/fphar.2021.787816(IF:5.811)

[34] Tang W, Zhao Y, Zeng J, et al. Integration of Small RNA and Transcriptome Sequencing Reveal the Roles of miR395 and ATP Sulfurylase in Developing Seeds of Chinese Kale. Front Plant Sci. 2022;12:778848. Published 2022 Feb 3. doi:10.3389/fpls.2021.778848(IF:5.754)

[35] Yu J, Mao C, Zhong Q, et al. OsNAC2 Is Involved in Multiple Hormonal Pathways to Mediate Germination of Rice Seeds and Establishment of Seedling. Front Plant Sci. 2021;12:699303. Published 2021 Jul 23. doi:10.3389/fpls.2021.699303(IF:5.754)

[36] Wu S, Guo X, Zhou J, et al. High expression of UNC5B enhances tumor proliferation, increases metastasis, and worsens prognosis in breast cancer [published online ahead of print, 2020 Sep 9]. Aging (Albany NY). 2020;12(17):17079-17098. doi:10.18632/aging.103639(IF:5.682)

[37] Zhu Z, Zhang Y, Wang X, Wang X, Ye SD. Inhibition of protein kinase D by CID755673 promotes maintenance of the pluripotency of embryonic stem cells. Development. 2020;147(16):dev185264. Published 2020 Aug 24. doi:10.1242/dev.185264(IF:5.611)

[38] Tang Q, Fu W, Zhang M, et al. Novel androgen receptor antagonist identified by structure-based virtual screening, structural optimization, and biological evaluation. Eur J Med Chem. 2020;192:112156. doi:10.1016/j.ejmech.2020.112156(IF:5.573)

[39] Sui Q, Chen Z, Hu Z, et al. Cisplatin resistance-related multi-omics differences and the establishment of machine learning models. J Transl Med. 2022;20(1):171. Published 2022 Apr 11. doi:10.1186/s12967-022-03372-0(IF:5.531)

[40] Yang Y, Chen R, Wu X, et al. Rapid and Efficient Conversion of Human Fibroblasts into Functional Neurons by Small Molecules. Stem Cell Reports. 2019;13(5):862-876. doi:10.1016/j.stemcr.2019.09.007(IF:5.499)

[41] Zhang XT, Yu YY, Xu HY, et al. Prevailing Role of Mucosal Igs and B Cells in Teleost Skin Immune Responses to Bacterial Infection. J Immunol. 2021;206(5):1088-1101. doi:10.4049/jimmunol.2001097(IF:5.422)

[42] Chen L, Ma Q, Zhang G, et al. Protective effect and mechanism of loganin and morroniside on acute lung injury and pulmonary fibrosis [published online ahead of print, 2022 Mar 5]. Phytomedicine. 2022;99:154030. doi:10.1016/j.phymed.2022.154030(IF:5.340)

[43] Wang Z, Nie K, Su H, et al. Berberine improves ovulation and endometrial receptivity in polycystic ovary syndrome. Phytomedicine. 2021;91:153654. doi:10.1016/j.phymed.2021.153654(IF:5.340)

[44] Wang X, Liu F, Cui Y, Yin Y, Li S, Li X. Apple Polyphenols Extracts Ameliorate High Carbohydrate Diet-Induced Body Weight Gain by Regulating the Gut Microbiota and Appetite. J Agric Food Chem. 2022;70(1):196-210. doi:10.1021/acs.jafc.1c07258(IF:5.279)

[45] Wang M, Guo W, Li J, et al. The miR528-AO Module Confers Enhanced Salt Tolerance in Rice by Modulating the Ascorbic Acid and Abscisic Acid Metabolism and ROS Scavenging. J Agric Food Chem. 2021;69(31):8634-8648. doi:10.1021/acs.jafc.1c01096(IF:5.279)

[46] Xue T, Lu Y, Yang H, et al. Isothermal RNA Amplification for the Detection of Viable Pathogenic Bacteria to Estimate the Salmonella Virulence for Causing Enteritis. J Agric Food Chem. 2022;70(5):1670-1678. doi:10.1021/acs.jafc.1c07182(IF:5.279)

[47] Zhang X, Li Y, Ji J, et al. Gadd45g initiates embryonic stem cell differentiation and inhibits breast cell carcinogenesis. Cell Death Discov. 2021;7(1):271. Published 2021 Oct 2. doi:10.1038/s41420-021-00667-x(IF:5.241)

[48] Gao Z, Gao X, Fan W, et al. Bisphenol A and genistein have opposite effects on adult chicken ovary by acting on ERα/Nrf2-Keap1-signaling pathway. Chem Biol Interact. 2021;347:109616. doi:10.1016/j.cbi.2021.109616(IF:5.194)

[49] Lin Y, Shen X, Ke Y, et al. Activation of osteoblast ferroptosis via the METTL3/ASK1-p38 signaling pathway in high glucose and high fat (HGHF)-induced diabetic bone loss. FASEB J. 2022;36(3):e22147. doi:10.1096/fj.202101610R(IF:5.192)

[50] Liu H, Wang LL, Xu QH, et al. UHRF1 shapes both the trophoblast invasion and decidual macrophage differentiation in early pregnancy. FASEB J. 2022;36(4):e22247. doi:10.1096/fj.202101647RR(IF:5.192)

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