Cloning refers to the insertion of nucleic acid into viruses, plasmids or other carrier molecules in vitro to form new combinations of genetic material that can enter the host cell and achieve sustained and stable proliferation and expression. It is widely used to study the function of various genes. The development of molecular cloning technology goes through three stages: The first is the classic T4 DNA ligase mediated TA cloning and sticky terminal cloning; the second is LIC series cloning based on DNA modification enzymes; the third is Gateway cloning and one-step cloning based on DNA recombinant enzymes. Yeasen provides complete solutions of these three princiles of cloning, you can contact us to obtain the guideline of choosing cloning methods.