V Universal Multiplex One Step RT-qPCR Probe Kit

Product Description

Hifair^TM V Multiplex One-Step RT-qPCR Probe Kit is for multiple quantitative PCR reactions with RNA as a template. During the experiment, the reverse transcription and quantitative PCR were carried out in the same reaction tube, simplified experimental operation and reduced the risk of pollution.

The kit uses heat-resistant Hifair^TM V Reverse Transcriptase to efficiently synthesize the first chain cDNA, and uses Unicon^TM Hotstart Taq DNA Polymerase to quantify quantitative amplification. The kit mainly includes an optimized MP Buffer, Enzymes Mix, etc., which already contains Mg2+ and DNTP, etc., and has added factors that effectively inhibit non-specific PCR amplification factors and enhance multiple qPCR reactive amplification efficiency, which can be guaranteed at the same time of primer amplification, up to four reactions.

Applicable Models

ABI Series: ABI 5700, 7000, 7300, 7700, 7900HT Fast, StepOne, StepOne Plus; 7500, 7500 Fast, ViiA7, QuantStudio 3 and 5, QuantStudio 6, 7, 12k Flex;

Bio-Rad Series: Bio-Rad CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4;

Roche Series: Roche Applied Science LightCycler 480, LightCycler 2.0; Lightcycler 96;

Others: Stratagene MX3000P, MX3005P, MX4000P;

Eppendorf Mastercycler ep realplex, realplex 2 s;

Qiagen Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000;

Thermo Scientific PikoReal Cycler; Cepheid SmartCycler; Illumina Eco qPCR.

Shipping and Storage

The product is shipped with ice packs and can be stored at -20°C for one year. Please avoid repeated freeze-thaw. It is recommended to save.

Cautions

1) Please use the RNase-free consumables during the experiment;

2) For your safety and health, please wear lab coats and disposable gloves for operation.