Viral vector, a virus based gene vector, is a common tool of molecular biology and can be used to deliver target genes into cultured cells or whole living organism. As one of the important materials of cell and gene therapy products, viral vector production is a complex process, which requires the plasmid with encoding virus and target gene to be transferred into tool cells in transfection reagents to produce viral vector with target gene, and then the required viral vector can be obtained through extraction, purification and filtration. Its production process can be divided into upstream virus amplification and downstream virus purification, canning.