Description

This product consists of 9 highly purified and prestained proteins ranging in molecular weight from 25 kDa to 300 kDa (25,45,72,100,130,160,200,250,300 kDa), among them, 72 kDa is an orange band, 25 kDa is a green band. The labeled apparent molecular weight was calibrated by the molecular weight Marker of standard non-prestained proteins. Using this product, the state of protein electrophoresis and the effect of membrane transfer can be dynamically observed. After SDS-PAGE electrophoresis, the color bands were transferred to the o PVDF membrane and NC membrane. This product is conveniently packaged and is a ready-to-use product, do not heat, dilute or add reducing agents! After the prestained protein is combined with the dye, in different buffer systems, there is displacement. There are signs in the instructions, this product is only for reference when judging the molecular weight of the target protein.

Features

• Bright color - three color predye, each strip average concentration of 2.5 μg
• Lower amount of this product into the gel hole - usually used for loading 5-10 μL each time
• Stable performance - 50℃ for 20 h, no degradation
• Long shelf life - can be stored at -30℃ ~ -15℃ for two years

Applications

• Protein migration is monitored during SDS-PAGE electrophoresis
• Monitor protein transfer to the membrane after Western blotting
• Protein size analysis on SDS-PAGE and Western blotting

Specifications

Product Type Specs	Protein Ladder
Size Range	25 kDa to 300 kDa
Number of Markers	9
Molecular Weight	25,45,72,100,130,160,200,250,300 kDa
Stain Type	72 kDa is orange band, 25 kDa is green band, the rest are blue bands
Storage Buffer	62.5 mM Tris-H3PO4 (pH 7.5), 2 mM EDTA, 2% (W/V) SDS, 33% (W/V) Glycerol, 5 mM DTT, 0.02% (V/V) proclin300
Ready to Load	ready-to-use product, do not heat, dilute or add reducing agents

Components

Components No.	Name	20347ES76	20347ES90
20347	GoldBand™ Plus 3-color High Range Protein Marker (25-300 kDa)	2×250 μL	10×250 μL

Storage

The product shouldbe stored at -25℃ ~ -15℃ for two years. For regular use, it can be placed at 4℃, valid for three months. It is recommended to store in aliquots to avoid repeated freezing and thawing!

Figures

Figure 1 7% SDS-PAGE electrophoresis results

 

Figure 2 Under different electrophoresis buffer conditions, the position of each band of this product