Murine RNase inhibitor GMP-grade (40 U/μL)

Details:

Description

RNase inhibitor can bind RNase to form a complex, thereby specifically inactivating RNase. This product is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form, which can inhibit various types of RNase (RNase A, B, C). Also tested by RT-PCR and RT-qPCR. Compared with human-derived RNase inhibitor, this product does not contain the two cysteines that are very sensitive to oxidation in human-derived proteins, so it has higher antioxidant activity; and it’s more suitable for high-DTT sensitivity experiments ( such as qPCR, etc.). This product is produced in accordance with GMP process requirements and is provided in liquid form.

Feature

  • Improved resistance to oxidation, compared to human/porcine RNase inhibitor
  • Ideal for reactions where low DTT concentrations are required (e.g., Real-time PCR)
  • Isolated from a recombinant source
  • Tested for the absence of DNases and RNases

Application

  • RT-PCR
  • cDNA synthesis
  • In vitro transcription/translation
  • Enzymatic RNA labeling reaction
  • Other applications where the integrity of RNA is important

Specification

Source Recombinant E.coli with Murine RNase inhibitor gene
Storage Buffer 20 mM Hepes KOH,150 mM KCl,8 mM DTT,0.5%(v/v)NP 40,0.5%(v/v)Tween20,50%(v/v)Glycerol,pH 7.5 ±0.2 (25℃)
Unit Definition The amount of enzyme required to inhibit 50% of the activity of 5 ng RNase A is defined as 1 activity unit

Components

Components No. Name 10621ES10 (10 KU) 10621ES20 (20 KU) 10621ES60 (100 KU) 10621ES99 (1 MU)
10621 Murine RNase inhibitor GMP-grade (40 U/μL) 250 μL 500 μL 2.5 mL 25 mL

Shipping and Storage

The Murine RNase inhibitor GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.

Citations & References:

[1] Shen W, Wang R, Fan Q, Li Y, Cheng Y. Natural polyphenol assisted delivery of single-strand oligonucleotides by cationic polymers. Gene Ther. 2020;27(7-8):383-391. doi:10.1038/s41434-020-0151-y(IF:4.128)

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