Fast blocking western is a new generation of ready-to-use, efficient, and fast Western blocking solutions. The blocking time is only 10 minutes, which is better than the traditional blocking solutions such as skim milk powder, BSA, and casein, and simplifies the Western blotting experiment. Blocking with Fast blocking western prevents nonspecific binding during antibody detection, but allows specific detection. Compared with blocking solutions, it has a higher signal, lower background, and higher signal-to-noise ratio.
The reagent does not contain protein components of mammalian origin to avoid cross reaction with antibodies. This buffer is compatible with phosphorylated antibodies as well as NC and PVDF membranes. But not for biotin labeled antibodies.
Features
Applications
Concentration | 1X |
Product Type | Blocking Buffer |
Validated Application | Western Blot |
Components No. | Name | 36122ES76 |
36122 | Fast Blocking Western | 500 mL |
The product should be stored at 2℃ ~ 8℃ for one year.
Figure 1 Compared with the skim milk powder, the blocking time is greatly shortened, only 1/6 of that. Compared with the competitor's blocking liquid, the strip was brighter and the background was lower.
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