MOPS, Free Acid

Product Description

3-(N-morpholino)propanesulfonic Acid, abbreviated as MOPS, is a zwitterionic buffer commonly used in biochemistry and molecular biology, and its structure is similar to 2-(N-morpholino)ethanesulfonic acid (MES). The effective pH buffer range of MOPS is 6.5-7.9, pKa=7.2 (25℃), and its pKa is closer to the physiological pH 7.4 than MES (pKa=6.1), so it is more suitable for use as a physiologically relevant buffer. For example, it is used for mammalian cell culture (concentration should be ≤ 20 mM), RNA denaturing agarose gel electrophoresis experiment (20 mM concentration is better for formaldehyde gel), discontinuous polyacrylamide gel electrophoresis, etc.

Product Nature

Synonym	3-(N- morpholino) propanesulfonic acid
CAS NO.	1132-61-2
Formula	C7H15NO4S
Molecular Weight	209.26 g/moL
Melting Point	277-280 ℃
Useful Buffering Range	pH 6.5-7.9 (25 ℃)
Appearance	White crystal powder
Purity	>99%
Solubility	Easily soluble in water (1 M), a transparent colorless solution
Structure

Shipping and Storage Methods

The product is transported and stored at room temperature and is valid for 1 year. On standing, the bulk powder may clump together and form hard lumps without affecting its chemical properties.
The MOPS solution can be stored at 2-8°C for at least 6 months. Filter sterilize with a 0.2 μm filter. Autoclaving is not recommended. To prepare a nuclease-free MOPS solution, autoclave the water before adding the powder to dissolve.

Cautions

1) For your safety and health, please wear lab coats and disposable gloves for operation.
2) For research use only!