DH5α Chemically Competent Cell

Description

DH5α Chemically Competent Cell from YEASEN is a competent cell obtained by a special process of DH5α strain. It can be used for the chemical transformation of DNA with a conversion efficiency of up to 10⁸ cfu/μg plasmid DNA. It is widely used for efficient DNA cloning and plasmid amplification and can ensure the stable inheritance of high-copy plasmids. At the same time, it can be used to construct clones and blue-white spot screening. Deletion of recA1 and endA1 genes in DH5α cells ensured the stability of cloned DNA.

DH5α competent cell genotypes: F- φ80 lac ZΔM15 Δ(lacZYA-arg F) U169 endA1 recA1 hsdR17(rk-,mk+) supE44λ- thi -1 gyrA96 relA1phoA

Features

• High transformation efficiency: >1×108 cfu/μg pUC19 DNA
• Genetic markers that allow for blue/white colony screening

Applications

• Efficient DNA cloning derived from PCR, cDNA-generation reactions
• recA1 marker facilitates working with difficult-to-transform DNA

Specifications

Species	Escherichia coil
Cell type	Chemical competent cell
Whether it contains an F’ episome	No
Efficiency	>1×10^8
Blue/white colony screening	Yes
Bacterial or yeast strains	DH5α
Amplification of the ccdB-containing vector	It is not suitable for the amplification of ccdB-containing vectors

Components

Components No.	Name	11802ES80	11802ES92
11802-A	DH5α Chemically Competent Cell	10×100 μL	100×100μL
11802-B	pUC19 (control vector,10 pg/μL)	10 μL	10 μL

Shipping and Storage

The product is shipped with dry ice and can be stored at -85℃~-65℃ for six months.

Do not store the product in -20℃ or liquid nitrogen.