UCF.METM High Affinity RNase Inhibitor (40 U/μL) is a recombinant porcine RNase inhibitor expressed in E. coli. It specifically inhibits the activity of RNases A, B and C through binding noncovalently. This product can be compatible with HifairTM Ultra Reverse Transcriptase (200 U/μL) (Cat#14604ES) and various DNA polymerases verified by RT-PCR and RT-qPCR. UCF.METM High Affinity RNase Inhibitor (40 U/μL) is lower with the purification process specially developed by YEASEN, which is suitable for application with more stringent requirements on background bacteria, such as pathogen microorganism detection.
Cat.No. |
14675ES05 / 14675ES20 / 14675ES60 |
Size |
2 KU / 20 KU / 100 KU |
Unit Definition |
The required amount of RNase Inhibitor to inhibit 50% activity of 5-ng RNase A is defined as one unit. The activity of RNase A is measured by hydrolyzing of cyclic 2', 3'-CMP to generate 3'-CMP. |
Name |
14675ES05 |
14675ES20 |
14675ES60 |
UCF.METM High Affinity RNase Inhibitor (40 U/μL) |
50 μL |
500 μL |
2.5 mL |
This product should be stored at -25~-15℃ for 2 years.
1. Add the following components to a nuclease-free microcentrifuge tube:
Components |
Volume (μL) |
RNase-free ddH2O |
to 20 |
5×HifairTM Ultra Reverse Transcriptase Reaction Buffer* |
4 |
Oligo (dT)18 (50 μM) |
1 |
dNTP Mix (10 mM each) |
1 |
UCF.METM High Affinity RNase Inhibitor (40 U/μL) |
0.5-1 |
HifairTM Ultra Reverse Transcriptase (200 U/μL)* |
1 |
RNA template |
Total RNA:1 ng-5 μg |
*According to the specific experimental application, 14604ES can be purchased for use together.
2.Thermal cycling protocol
Temperature |
Time |
50℃* |
15 min |
85℃** |
5 min |
*Reverse transcription temperature: When used with 14604ES, 50℃ is recommended; For high GC content templates or complex templates, the reverse transcription temperature can be increased to 55℃-60℃.
**inactivate the reaction by heating at 85℃ for 5 min.
3. The products of reverse transcriptase reaction can be immediately used for subsequent PCR or qPCR reactions, and can be stored at -25~-15℃ for short-term storage. If long-term storage is required, it is recommended to store them at -80℃ after packaging to avoid repeated freeze-thaw products.
1. This product is for scientific research purposes only.
2. Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety.
Ver. EN20230921
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