Product description

The Blood Advanced Direct PCR Kit is a kit that enables direct PCR amplification of whole blood samples without DNA purification or sample pretreatment. This kit is compatible with fresh blood containing conventional anticoagulants such as EDTA, heparin, citrate, frozen blood and commercial Whatman903^TM and FTA^TM dried blood stains. The kit contains a combination of genetically engineered DNA Polymerase with high fidelity and tolerance to PCR inhibitors. It can efficiently amplify 8 kb genomic fragments under rapid elongation conditions, for the fragments less than 2 kb, the extension time of 3-5 sec/kb can be set to complete the amplification, thus significantly reducing the identification and detection time of blood samples.

 

Hieff^TM Advanced High-Fidelity DNA Polymerase Mix has a flat 3 'end of the amplified product, which is suitable for one-step rapid cloning and TOPO cloning (YEASEN Cat#10909/10910/10911/10922).

 

The Positive control primer mix (10 μmol/L each) provided in the kit is capable of amplifying a fragment 237 bp in length from the upstream conserved sequence of the sox21 gene in mammals and most vertebrates, which could be used as a positive control.

 

 

Features

●.Rapid expansion.

●.High GC compatibility.

●.Template tolerance is strong.

●.Wide applicability of species.

 

Applications

●.Direct PCR reaction of different types of whole blood samples.

 

Specifications

Product specification	Kit
Hot Start	Built-in Hot Start
Overhang	Blunt
Conditions for transportation	Ice Packs
Product Type	Direct PCR Kit
Apply to (application)	Different types of whole blood samples

Components

Components No.	Name	10188ES20	10188ES50	10188ES60	10188ES76
10188-A	2× HieffTM Blood Advanced PCR Buffer	500 μL	1.25 mL	2.5 mL	12.5 mL
10188-B	HieffTM Advanced High-Fidelity DNA Polymerase Mix	20 μL	50 μL	100 μL	500 μL
10188-C	Positive control primer mix (10 μM each)	50 μL	100 μL	200 μL	1 mL
10188-D	10× DNA loading buffer	200 μL	500 μL	1 mL	5× 1 mL

Storage

The products should be stored at -25~-15℃ for one years. Please avoid repeated freeze-thaw.

Ver.EN20231030