The protocol of Ulcerative Colitis Mice Modeling using Dextran sodium sulfate (DSS)

The mouse model is the most common model for colitis modeling. After the hamster ulcerative colitis model was first prepared by using dextran sulfate sodium (DSS) in 1985, there are a lot of data to prove the etiology and clinical characteristics of the DSS colitis model. Symptoms, pathological changes, and treatment responses are similar to those of human ulcerative colitis (UC) ^[1].

 

1. Protocol

2. Links to this resource

3. Successful case for modeling with Yeasen DSS

4. Product order

5. Published articles with our reagents

 

1. Protocol

1.1 Acute modeling

1) Sterile drinking water with 3%-5% DSS, and filter with a 0.22 μm membrane, the solution must be colorless or slightly yellowish and clear.
2) Mice are housed in groups, at least 6 mice per cage, and the animals are maintained under standard conditions (with a temperature of 25℃, a 12-h/12-h light/dark cycle, and ad libitum access to food and water)
3) The body weight, diarrhea, and blood in the stool of the mice were recorded every 24 hours.
4) After rearing for a week, the DSS aqueous solution was replaced with an ordinary DSS-free aqueous solution, and the index was finally recorded ^[2].

1.2 Chronic modeling

1) Prepare 1%-3% DSS sterile aqueous solution, and the operation is the same as that of acute modeling.
2) Same as acute modeling 2)
3) Same as acute modeling 3)
4) Drink freely for several weeks, and finally replace the DSS aqueous solution with an ordinary DSS-free aqueous solution, and finally record the indicators ^[3].

Figure 1. Modeling scheme of acute and chronic colitis in mice

1.3 Results of a successful IBD model

Figure 2. HE staining results of DSS acute colitis sections^[4]

 

2. Links to this resource

[1] Ohkusa T. Production of experimental ulcerative colitis in hamsters by dextran sulfate sodium and changes in intestinal micro-flora[J]. Nihon Shokakibyo Gakkai Zasshi. 1985;82:1327-1336.
[2] Xiaona Gao a,b , Wentao Fan a,b , Lei Tan,etc.Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J]. J Nutr Biochem 2020 09;83.
[3] Li Y,  Dong J,  Xiao H, etc.Gut commensal derived-valeric acid protects against radiation injuries[J]. Gut Microbes 2020 07 03;11(4).
[4] Gao X, Fan W, Tan L, et al. Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J]. The Journal of Nutritional Biochemistry, 2020, 83.

 

3. Successful case for modeling with Yeasen DSS

Yeasen provides high-quality DSS (Cat#60316ES): purity (98%), sulfur content 17-19%, free sulfur <0.2%, supported by a large number of literature data.

Table 1. Construction of different types of enteritis models with DSS

Model	Modeling samples	Modeling plan	Modeling results	Use evaluation
acute colitis	BALB/c mice, female, 6-8 weeks, 25 g	3%-5% DSS drink freely for 7 consecutive days	Day 5 appeared, the length of the colon was shortened, HE staining, and the inflammation was obvious molding	molding speed is fast and the time is short. Consistent with the characteristics of acute colitis model
	C57BL/6 mice, male, 8 weeks, 20 g	3%-5% DSS by gavage, continuous administration	Day 5 occurs, colon shortening, weight loss, blood in the stool, diarrhea	High mold rate, and short duration. Consistent with the characteristics of acute colitis model
chronic colitis	C57BL/6 mouse, male, 8 weeks, 22 g	1-2% DSS by gavage, continuous administration	Day40 appears, colon shortening, weight loss, blood in the stool, diarrhea	High molding rate . Consistent with the characteristics of the chronic colitis model
colon cancer	C57BL/6 mouse, male, 8 weeks, 21 g	1%-2% DSS ad libitum for 5 days for 3 weeks	14 weeks with shortened colon length, weight loss, HE staining, and obvious inflammation	High molding rate . Consistent with colon cancer model characteristics

 

4. Product order

Hot-selling product only needs 1/3 of the price of M* with the same efficiency， and we keep a large stock.

Table 2. Product Order

Product Name	Article Number	Specification
Dextran sulfate sodium salt for modeling colitis MW: 36000~50000	60316ES25	25g
Dextran sulfate sodium salt for modeling colitis MW: 36000~50000	60316ES60	100 g
Dextran sulfate sodium salt for modeling colitis MW: 36000~50000	60316ES76	500 g
Dextran sulfate sodium salt for modeling colitis MW: 36000~50000	60316ES80	1 kg

 

5. Published articles with our reagents

[1] Li Zhao, Fei Wang, Zhengwei Cai, et al.Improving drug utilization platform with injectable mucoadhesive hydrogel for treating ulcerative colitis[J]. chemical engineering journal.424(2021)130464.IF=16.744
[2] Lingjun Tong, Haining Hao, Zhe Zhang, et al.Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota[J].Theranostics.2021; 11(17): 8570-8586 IF=11.556
[3] Li, Y., Dong, J., Xiao, H., Zhang, S., Wang, B., Cui, M., & Fan, S. Gut commensal derived-valeric acid protects against radiation injuries. Gut Microbes,.2020 .1–18.IF=10.245
[4] Jingjing Gan, Yuxiao Liu, Lingyu Sun, et al.Orally administrated nucleotide-delivery particles from microfluidics for inflammatory bowel disease treatment[J]. Applied Materials Today.2021 Dec;25:101231 IF=10.041
[5] Mengmeng Xu, Ying Kong, Nannan Chen,et al.Identification of Immune-Related Gene Signature and Prediction of CeRNA Network in Active Ulcerative Colitis[J].Frontiers in Immunology.2022; 13: 855645. IF=7.561
[6] JialiDong, YuanLi,HuiwenXiao, et al.Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models[J].Cell reports.2021, 109886.IF=9.423
[7] Hao H,  Zhang X,  Tong L,  Liu Q, et al.Lactobacillus plantarumEffect of Extracellular Vesicles Derived From  Q7 on Gut Microbiota and Ulcerative Colitis in Mice[J].Frontiers in Immunology.2021.777147 .IF=7.561
[8] Yaohua Fan, Yanqun Fan, Kunfeng Liu, et al.Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance[J].Frontiers in Pharmacology.2021.632602.IF=7.561
[9] Jia-Rong Huang, Sheng-Te Wang, Meng-Ning Wei, et al.Piperlongumine Alleviates Mouse Colitis and Colitis-Associated Colorectal Cancer[J].Frontiers in Pharmacology.2020.586885. IF=7.561
[10] Gao X, Fan W, Tan L, et al. Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J]. The Journal of Nutritional Biochemistry, 2020, 83.IF=6.048
[11] Lujuan Xing, Lijuan Fu, Songmin Cao,et al.The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice[J]. Nutrients 2022, 14, 1479. IF=5.717
[12] Wang S,  Huang J,  Tan KS, et al.Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-B Pathway[J].Oxidative Medicine and Cellular Longevity. 2022,4636618. IF=5.076