Hieff™ miRNA Universal qPCR SYBR Master Mix (Stem loop method)

Details:

Product Description

HieffTM miRNA Universal qPCR SYBR Master Mix is a premix for 2×real-time quantitative PCR amplification with high sensitivity and specificity, and quantitative PCR can obtain good linear relationship in a wide quantitative area. The HieffTM miRNA Taq DNA polymerase, a core component, was thermally initiated by antibody method and combined with an optimized buffer to effectively inhibit non-specific amplification caused by primer annealing during sample preparation. In addition, ROX Reference Dye is added to the premixed liquid system, which can be applied to different qPCR instruments. When configuring the reaction system, only the primer and template can be added for amplification.

Shipping and Storage

The components are shipped with ice packs and can be stored at -20°C for 18 months.

Please avoid repeated freeze-thaw. The product contains fluorescent dye SYBR Green I, which should be avoided when storing or preparing the reaction system.

Cautions

1. Recommended for use with our HifairTM III 1st Strand cDNA Synthesis Kit (gDNA digester plus) (Cat#11139).

2. Master Mix components should be fully shaken before use, centrifugal at low speed.

3. For your safety and health, please wear lab coats and disposable gloves for operation.

4. This product is for research use ONLY!

Citations & References:

[1] Chen T, Lei S, Zeng Z, et al. MicroRNA‑137 suppresses the proliferation, migration and invasion of cholangiocarcinoma cells by targeting WNT2B. Int J Mol Med. 2020;45(3):886-896. doi:10.3892/ijmm.2020.4474(IF:4.101)

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