Murine RNase Inhibitor is purified from a recombinant strain of E. coli in a soluble form. It specifically inhibits the activity of RNases A, B and C through binding noncovalently in a 1:1 ratio with high affinity. Recombinant murine RNase inhibitor does NOT contain 2 oxidation-sensitive cysteine which are contained in human-origin RNase inhibitor. Therefore, murine RNase inhibitor has high anti-oxidation activity and is more stable for low DTT experiments (< 1mmol/L). This product is validated for its compatibility with HifairTM Ⅱ Reverse Transcriptase (Cat#11110) and various DNA Polymerases. Murine RNase inhibitor is ideal for high-DTT-sensitive experiments, such as RT-PCR. The product can be used in cDNA Synthesis, polysome isolation and In vitro transcription/translation.
|Product Type||RNase Inhibitor|
|Unit Definition||The required amount of RNase Inhibitor to inhibit 50% activity of 5-ng RNase A is defined as one unit. The activity of RNase A is measured by hydrolyzing of cyclic 2', 3'-CMP to generate 3'-CMP.|
|Components No.||Name||10603ES05 (2 KU)||10603ES10 (10 KU)||10603ES20 (20 KU)||10603ES60 (100 KU)||10603ES94 (20,000 KU)|
|10603||Murine RNase Inhibitor (40 U/µL)||50 μL||250 μL||500 μL||2.5 mL||500 mL|
The product is shipped with dry ice and can be stored at -20°C for 2 years.
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