Murine RNase inhibitor (40 U/μL)

Details:

Description

Murine RNase Inhibitor is purified from a recombinant strain of E. coli in a soluble form. It specifically inhibits the activity of RNases A, B and C through binding noncovalently in a 1:1 ratio with high affinity. Recombinant murine RNase inhibitor does NOT contain 2 oxidation-sensitive cysteine which are contained in human-origin RNase inhibitor. Therefore, murine RNase inhibitor has high anti-oxidation activity and is more stable for low DTT experiments (< 1mmol/L). This product is validated for its compatibility with HifairTM Ⅱ Reverse Transcriptase (Cat#11110) and various DNA Polymerases. Murine RNase inhibitor is ideal for high-DTT-sensitive experiments, such as RT-PCR. The product can be used in cDNA Synthesis, polysome isolation and In vitro transcription/translation.

Features

  • Performs under a wide range of reaction conditions
  • Protects RNA from degradation at temperatures up to 55°C

Specifications

Concentration 40 U/μL
Product Type RNase Inhibitor
Unit Definition The required amount of RNase Inhibitor to inhibit 50% activity of 5-ng RNase A is defined as one unit. The activity of RNase A is measured by hydrolyzing of cyclic 2', 3'-CMP to generate 3'-CMP.

Components

Components No. Name 10603ES05 (2 KU) 10603ES10 (10 KU) 10603ES20 (20 KU) 10603ES60 (100 KU) 10603ES94 (20,000 KU)
10603 Murine RNase Inhibitor (40 U/µL) 50 μL 250 μL 500 μL 2.5 mL 500 mL

Shipping and Storage

The product is shipped with dry ice and can be stored at -20°C for 2 years.

Citations & References:

[1] Qiu J, Fan Q, Xu S, et al. A fluorinated peptide with high serum- and lipid-tolerence for the delivery of siRNA drugs to treat obesity and metabolic dysfunction. Biomaterials. 2022;285:121541. doi:10.1016/j.biomaterials.2022.121541(IF:12.479)

[2] Song Y, Guo Y, Li X, et al. RBM39 Alters Phosphorylation of c-Jun and Binds to Viral RNA to Promote PRRSV Proliferation. Front Immunol. 2021;12:664417. Published 2021 May 17. doi:10.3389/fimmu.2021.664417(IF:7.561)

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