Description

Murine RNase Inhibitor is purified from a recombinant strain of E. coli in a soluble form. It specifically inhibits the activity of RNases A, B and C through binding noncovalently in a 1:1 ratio with high affinity. Recombinant murine RNase inhibitor does NOT contain 2 oxidation-sensitive cysteine which are contained in human-origin RNase inhibitor. Therefore, murine RNase inhibitor has high anti-oxidation activity and is more stable for low DTT experiments (< 1mmol/L). This product is validated for its compatibility with HifairTM Ⅱ Reverse Transcriptase (Cat#11110) and various DNA Polymerases. Murine RNase inhibitor is ideal for high-DTT-sensitive experiments, such as RT-PCR. The product can be used in cDNA Synthesis, polysome isolation and In vitro transcription/translation.

Features

• Performs under a wide range of reaction conditions
• Protects RNA from degradation at temperatures up to 55°C

Specifications

Concentration	40 U/μL
Product Type	RNase Inhibitor
Unit Definition	The required amount of RNase Inhibitor to inhibit 50% activity of 5-ng RNase A is defined as one unit. The activity of RNase A is measured by hydrolyzing of cyclic 2', 3'-CMP to generate 3'-CMP.

Components

Components No.	Name	10603ES05 (2 KU)	10603ES10 (10 KU)	10603ES20 (20 KU)	10603ES60 (100 KU)	10603ES94 (20,000 KU)
10603	Murine RNase Inhibitor (40 U/µL)	50 μL	250 μL	500 μL	2.5 mL	500 mL

Shipping and Storage

The product is shipped with dry ice and can be stored at -20°C for 2 years.

Figure

Figure 1. RNase inhibitor can effectively inhibit RNase A activity to prevent the digestion of total RNA from HEK 293 cells.

Table 1. Examination of the inhibition of MRI on RNase A. Different concentration gradients of MRI were incubated with 100 ng of RNase A to block the activity of RNase A, followed by digestion with 1 μg of RNA and examined by one step RT-qPCR.