Product Information

Product Description

Hieff NGS™ DNA Selection Beads are prepared based on the SPRI (Solid Phase Reverse Immobilization) principle and is applicable for DNA purification and size selection during the preparation of next generation sequencing (NGS) libraries. Hieff NGS® DNA Selection Beads are compatible with various of DNA and RNA library prep kits. The method is exactly the same as the currently widely used AMPure XP Beads. The yield and size distribution of the library are highly consistent with AMPure XP Beads.

The Operation Flowchart of Size Selection

Figure 1 The Operation Flowchart of Size Selection

Recommended Conditions for DNA Size Selection

Table 1. Recommended condition for DNA size selection

Note: "×" in the table indicates the volume of sample DNA. For example, if the insert length of the library is 250 bp and the sample DNA volume is 100 μL, the volume of magnetic beads used in the first round of sorting is 0.80×100 μL=80 μL; the volume of magnetic beads used in the second round of sorting is 0.20× 100 μL=20 μL.

Figure 2. Agilent 2100 high sensitivity DNA chip electropherogram

Shipping and Storage

The beads are shipping with ice packs and can be stored at 2-8°C for one year.

Cautions

1. For your safety and health, please wear lab coats and disposable gloves for operation.

2. For research use only!