Hieff NGS™ DNA Selection Beads are prepared based on the SPRI (Solid Phase Reverse Immobilization) principle and is applicable for DNA purification and size selection during the preparation of next generation sequencing (NGS) libraries. Hieff NGS™ DNA Selection Beads are compatible with various of DNA and RNA library prep kits. The method is exactly the same as the currently widely used AMPure XP Beads. The yield and size distribution of the library are highly consistent with AMPure XP Beads.
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Product Information
Product Name | Cat# | Specification |
12601ES08 | 5 mL | |
Hieff NGS™ DNA Selection Beads | 12601ES56 | 60 mL |
12601ES75 | 450 mL |
Product Description
Hieff NGS™ DNA Selection Beads are prepared based on the SPRI (Solid Phase Reverse Immobilization) principle and is applicable for DNA purification and size selection during the preparation of next generation sequencing (NGS) libraries. Hieff NGS® DNA Selection Beads are compatible with various of DNA and RNA library prep kits. The method is exactly the same as the currently widely used AMPure XP Beads. The yield and size distribution of the library are highly consistent with AMPure XP Beads.
The Operation Flowchart of Size Selection
Figure 1 The Operation Flowchart of Size Selection
Recommended Conditions for DNA Size Selection
Table 1. Recommended condition for DNA size selection
Length of DNA fragment | 250-350 bp | 320-420 bp | 450-550 bp | 550-700 bp | 700-900 bp | 800-1,000 bp |
Ratio of Beads: DNA for the 1st Round | 0.80× | 0.70× | 0.60× | 0.55× | 0.50× | 0.45× |
Ratio of Beads: DNA for the 1st Round | 0.20× | 0.20× | 0.20× | 0.15× | 0.15× | 0.15× |
Note: "×" in the table indicates the volume of sample DNA. For example, if the insert length of the library is 250 bp and the sample DNA volume is 100 μL, the volume of magnetic beads used in the first round of sorting is 0.80×100 μL=80 μL; the volume of magnetic beads used in the second round of sorting is 0.20× 100 μL=20 μL.
Figure 2. Agilent 2100 high sensitivity DNA chip electropherogram
Shipping and Storage
The beads are shipping with ice packs and can be stored at 2-8°C for one year.
Cautions
1. For your safety and health, please wear lab coats and disposable gloves for operation.
2. For research use only!