DNA selection Beads(Superior Ampure XP alternative)

Hieff NGS™ DNA Selection Beads are prepared based on the SPRI (Solid Phase Reverse Immobilization) principle and is applicable for DNA purification and size selection during the preparation of next generation sequencing (NGS) libraries. Hieff NGS™ DNA Selection Beads are compatible with various of DNA and RNA library prep kits. The method is exactly the same as the currently widely used AMPure XP Beads. The yield and size distribution of the library are highly consistent with AMPure XP Beads.

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Product Information

Product Name

Cat#

Specification

12601ES08

5 mL

Hieff NGS DNA Selection Beads

12601ES56

60 mL

12601ES75

450 mL

Product Description

Hieff NGS DNA Selection Beads are prepared based on the SPRI (Solid Phase Reverse Immobilization) principle and is applicable for DNA purification and size selection during the preparation of next generation sequencing (NGS) libraries. Hieff NGS® DNA Selection Beads are compatible with various of DNA and RNA library prep kits. The method is exactly the same as the currently widely used AMPure XP Beads. The yield and size distribution of the library are highly consistent with AMPure XP Beads.

The Operation Flowchart of Size Selection


Figure 1 The Operation Flowchart of Size Selection

Recommended Conditions for DNA Size Selection

Table 1. Recommended condition for DNA size selection

Length of DNA fragment

250-350 bp

320-420 bp

450-550 bp

550-700 bp

700-900 bp

800-1,000 bp

Ratio of Beads:

DNA for the 1st Round

0.80×

0.70×

0.60×

0.55×

0.50×

0.45×

Ratio of Beads:

DNA for the 1st Round

0.20×

0.20×

0.20×

0.15×

0.15×

0.15×

Note: "×" in the table indicates the volume of sample DNA. For example, if the insert length of the library is 250 bp and the sample DNA volume is 100 μL, the volume of magnetic beads used in the first round of sorting is 0.80×100 μL=80 μL; the volume of magnetic beads used in the second round of sorting is 0.20× 100 μL=20 μL.


Figure 2. Agilent 2100 high sensitivity DNA chip electropherogram

Shipping and Storage

The beads are shipping with ice packs and can be stored at 2-8°C for one year.

Cautions

1. For your safety and health, please wear lab coats and disposable gloves for operation.

2. For research use only!