Ampicillin, Sodium Salt



Ampicillin is a β-lactam antibiotic within the penicillin family. As a member of this family, Ampicillin is susceptible to β-lactamase, which hydrolyzes the β-lactam ring. This broad-spectrum antibiotic is effective against gram-positive, gram-negative bacteria, and anaerobic bacteria. As an antibiotic, Ampicillin binds to penicillin-binding proteins (PBPs), acting as a structural analog of acyl-D-alanyl-D-alanine, acylating the transpeptidase enzyme, and thereby preventing the cross-linking of the peptidoglycan of the cell wall necessary for the growth of the bacterium. Ampicillin in the form of sodium salt from Yeasen, as a selective agent in E.coli culture, is widely used to prepare LB medium.

As a selective agent in E.coli culture, Ampicillin is widely used to prepare LB medium. The minimum inhibitory concentration (MIC) of ampicillin against Gram-negative bacteria and Gram-positive bacteria were 0.03-3 μg/mL and 0.02-1.5 μg/mL, respectively.   Agar or culture medium for autoclave should be cooled to 45-50°C before adding ampicillin solution. A culture medium containing ampicillin can be stored at 2-8°C for 2 weeks, and at 37°C for approximately 3 days.

This product is ampicillin sodium salt. The recommended concentration for cell screening is 50-100 μg/mL.


  • High-quality raw materials, all antibiotic product raw materials are sourced from high-quality fixed suppliers
  • Standardized production, using factory mass production mode
  • Wide range of applications, which can be used in the fields of molecular biology and biochemical experimental research of tissue culture
  • The cooperation platform covers the whole
  • To ensure product quality stability, the difference between batches is controlled within 1%


  • As a selective agent in E.coli culture, Ampicillin is widely used to prepare LB medium
  • Used for sterilization in cell culture


Synonyms D-(-)-α-Aminobenzylpenicillin sodium salt, Amblosin, Plumericin, Viacilina-A
CAS# 69-52-3
Formula C16H18N3NaO4S
Molecular Weight 371.39
Appearance White to off-white powder
Potency (Anhydrous) 845-988 mcg/mg
Solubility Soluble in water: ≥ 50 mg/mL


Components No. Name 60203ES10 60203ES60
60203 Ampicillin, Sodium Salt 10 g 100 g

Shipping and Storage

The product is shipped with ice packs and can be stored at 2℃ ~ 8℃ for three years.


Figure 1 The result of the bacteriostasis test

Type of Bacteria: E.coli

Usage amount: 5 µg/mL, 10 µg/mL, 25 µg/mL, 50 µg/mL
Citations & References:

[1] Zou Y, Wang A, Shi M, et al. Analysis of redox landscapes and dynamics in living cells and in vivo using genetically encoded fluorescent sensors. Nat Protoc. 2018;13(10):2362-2386. doi:10.1038/s41596-018-0042-5(IF:12.423)

[2] Wu R, Mei X, Ye Y, et al. Zn(II)-curcumin solid dispersion impairs hepatocellular carcinoma growth and enhances chemotherapy by modulating gut microbiota-mediated zinc homeostasis. Pharmacol Res. 2019;150:104454. doi:10.1016/j.phrs.2019.104454(IF:7.658)

[3] Ma H, Lin J, Li L, et al. Formaldehyde reinforces pro-inflammatory responses of macrophages through induction of glycolysis. Chemosphere. 2021;282:131149. doi:10.1016/j.chemosphere.2021.131149(IF:7.086)

[4] Zhang X, Tang W, Wen H, et al. Evaluation of CTB-sLip for Targeting Lung Metastasis of Colorectal Cancer. Pharmaceutics. 2022;14(4):868. Published 2022 Apr 15. doi:10.3390/pharmaceutics14040868(IF:6.321)

[5] Jin M, Kong L, Han Y, Zhang S. Gut microbiota enhances the chemosensitivity of hepatocellular carcinoma to 5-fluorouracil in vivo by increasing curcumin bioavailability. Phytother Res. 2021;35(10):5823-5837. doi:10.1002/ptr.7240(IF:5.882)

[6] Li Z, Zhang L, Song Q, et al. Proteomics Analysis Reveals Bacterial Antibiotics Resistance Mechanism Mediated by ahslyA Against Enoxacin in Aeromonas hydrophila. Front Microbiol. 2021;12:699415. Published 2021 Jun 8. doi:10.3389/fmicb.2021.699415(IF:5.640)

[7] Qiu Y, Yu J, Li Y, et al. Depletion of gut microbiota induces skeletal muscle atrophy by FXR-FGF15/19 signalling. Ann Med. 2021;53(1):508-522. doi:10.1080/07853890.2021.1900593(IF:4.709)

[8] Tan F, Limbu SM, Qian Y, Qiao F, Du ZY, Zhang M. The Responses of Germ-Free Zebrafish (Danio rerio) to Varying Bacterial Concentrations, Colonization Time Points, and Exposure Duration. Front Microbiol. 2019;10:2156. Published 2019 Sep 18. doi:10.3389/fmicb.2019.02156(IF:4.259)

[9] Yang Y, Yu S, Liu N, et al. Transcription Factor FOXO3a Is a Negative Regulator of Cytotoxicity of Fusarium mycotoxin in GES-1 Cells. Toxicol Sci. 2018;166(2):370-381. doi:10.1093/toxsci/kfy216(IF:4.181)

[10] Ji X, Han T, Kang N, Huang S, Liu Y. Preparation of RGD4C fused anti-TNFα nanobody and inhibitory activity on triple-negative breast cancer in vivo. Life Sci. 2020;260:118274. doi:10.1016/j.lfs.2020.118274(IF:3.647)
[11] Liao PC, Franco-Iborra S, Yang Y, Pon LA. Live cell imaging of mitochondrial redox state in mammalian cells and yeast. Methods Cell Biol. 2020;155:295-319. doi:10.1016/bs.mcb.2019.11.008(IF:1.441)

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