Description

TOP10 Chemically Competent Cell from YEASEN is the competent cells obtained by the special process of Escherichia coli strain TOP10 with Streptomycin (StrR) resistance. It can be used for the chemical transformation of DNA with a conversion efficiency of up to 10⁸ cfu/μg plasmid DNA. It is widely used for efficient DNA cloning and plasmid amplification, and can ensure stable inheritance of high-copy plasmids. At the same time, it can be used to construct clones and blue-white colony screening.

TOP10 competent cell genotypes: F-mcrA △(mrr-hsdRMS-mcrBC) φ80lacZ△M15 △lacX74 recA1 ara△139 △(ara-leu) 7697 galU galK rpsL (StrR ) endA1 nupG.

Features

• High transformation efficiency: >1×10⁸ cfu/μg pUC19 DNA
• recA1 was used to reduce nonspecific recombination in cloned DNA
• lacZΔM15 was used for blue/white colony screening of recombinant clones

Applications

• Efficient cloning
• Plasmid proliferation

Specifications

Species	Escherichia coil
Cell type	Chemical competent cell
Whether it contains an F’ episome	No
Efficiency	>1×10^8
Blue-white colony screening	Yes
Bacterial or yeast strains	TOP10
Amplification of the ccdB-containing vector	It is not suitable for the amplification of ccdB-containing vectors

Components

Components No.	Name	11801ES80	11801ES92
11801	TOP10 Chemically Competent Cell	10×100 μL	100×100μL

Shipping and Storage

The product is shipped with dry ice and can be stored at -85℃~-65℃ for six months.

Do not store the product in -20℃ or liquid nitrogen.