Description

Anti-DYKDDDDK (Flag) Affinity Gel is prepared by conjugating high quality murine IgG2b monoclonal antibody with 4% highly cross-linked agarose gel. This product has a high Flag label fusion protein loading capacity (at least 1.1 mg protein/mL gel) and less non-specific binding of miscellaneous proteins. It can be used for immunoprecipitation (IP) and purification of fusion proteins with Flag labels.

Anti-DYKDDDDK (FLAG) Affinity Gel can bind to the Met modified N-flag fusion Protein (MET-flag-Protein), N-flag fusion protein (Flag-protein), C-terminal FLAG Fusion Protein (protein-flag).

Features

High Specificity—The selection of high quality murine IgG2b monoclonal antibody can specifically adsorb the Flag  protein sequence.

high yield and high purity—Obtain high purity Flag label protein

High binding capacity—Capacity ≥1.1mg protein /mL gel

Many types of protein binding—Met-flag-Protein, N-flag fusion protein, C-terminal FLAG Fusion Protein

Widely used—Can be used for protein purification or IP

Applications

Immunoprecipitation (IP)

Purification of fusion proteins with Flag labels.

Specifications

Clone	1A3
Isotype	Mouse IgG2b
Application	Protein purification、IP
Protein binding capacity	≥1.1 mg protein/mL Gel
Storage buffer	TBS，50% glycerin，pH7.4，contains 0.02% (w/v) sodium azide

Components 

Components No.	Name	20584ES03	20584ES08	20584ES25	20584ES60
20584	Anti-DYKDDDDK (Flag) Affinity Gel	1 mL	5 mL	25 mL	100 mL

Note: In order to facilitate customers to use 1×Flag polypeptide elution, this product package is accompanied by 1×Flag polypeptide (Cat#20572ES).

Based on the different specifications of 1×Flag polypeptide (Cat#20572ES), 1 mL gel package is combined with 5 mg peptide, and other gel specifications are combined with 1 mL: 1 mg ratio of 5 mg specification 1×Flag polypeptide (Cat#20572ES).

Shipping and Storage

The products are shipped with ice pack, and unopened products can be stably stored at -25~-15℃ for 1 year. Do not freeze in solution without glycerin!

Figures

Figure 1. The SDS-PAGE gel electrophoresis results of Protein purification with Anti-Flag Affinity Gel (20584ES). 1: electrophoresis bands were purified by adding enterobacter lysate 2: negative control

Figure 2. Purification of low expression protein by  Anti-Flag affinity gel (20584ES) Affinity Gel. a：Flag-N    b：Flag-M    c：Flag-C M：Protein Marker 1：Flag-C pre-column stock solution 2：Flag-CPost-column penetrating solution 3: elution (pH2.7)  4: elution (pH12 ) 5: elution (1×Flag polypeptide )  6: purified gel (pH2.7 elution) 7: purified gel (pH12 elution) 8: Purified gel (1×Flag polypeptide elution)