Product description

2× Hieff Canace™ AdvanceFast PCR Master Mix is a ready-to-use 2× pre-mixed solution containing Hieff Canace™ AdvanceFast High-Fidelity DNA Polymerase, dNTPs, and an optimized buffer system. The amplification products are flat ends. 2× Hieff Canace™ AdvanceFast PCR Master Mix has the advantages of quick and easy, high sensitivity, high specificity, good stability, etc., the reaction system can be added with only the primers and templates. In addition, the product also contains a specific protective agent, so that the premix can still maintain stable activity after repeated freezing and thawing.

Specification

Cat.No.	10163ES01/10163ES03/10163ES08
Size	250 μL/1 mL/5×1 mL

Storage

This product should be stored at -25~-15℃ for 1 years.

Instructions

1. Recommended PCR reaction systems.

Components	Volume（μL）	Final concentration
2× Hieff Canace™ AdvanceFast PCR Master Mix*	25	1×
Template**	x	-
Forward Primer（10 μmol/L）***	2	0.4 μmol/L
Reverse Primer（10 μmol/L）	2	0.4 μmol/L
ddH2O	Up to 50	-

Table 1 PCR reaction system

*In 1× premixes containing 2 mmol/L Mg²⁺ and 200 μmol/L dNTPs.

**Recommended range 10-200 ng, cDNA sample upload volume range not more than 1/10 of the reaction system, recommended 1-2.5 μL.

***The final primer concentration in the PCR reaction system ranges from 0.2-1 μmol/L, and 0.4 μmol/L is recommended.

2. Reaction program.

Cycle step	Temp.	Time	Cycles
Initial denaturation	98℃	30 sec	1
Denaturation	98℃	10 sec	30-35
Annealing*	60℃	5 sec
Extension**	72℃	5-10 sec/kb
Final extension	72℃	2 min	1

Table 2 PCR reaction program

*Recommended temperature: 60°C, a temperature gradient can be set up to find the optimal temperature for primer annealing. The recommended annealing time is set to 5 sec and can be adjusted from 5-30 sec. Too long annealing time may result in diffuse amplification products on the gel.

**Extension time: Recommended 5 sec/kb, can also be extended to 10 sec/kb as needed.

Notes

 

1. This product is for research use only.

2. Please operate with lab coats and disposable gloves，for your safety.

Ver.EN20230919