Bst Ⅱ Plus DNA Polymerase (40 U/μL)

Bst DNA polymerase is the core enzyme of LAMP isothermal amplification technology. It has strong thermal stability, strand displacement activity and polymerase activity. Bst II Plus DNA Polymerase (40 U/μL) provided by Yeasen biotech is obtained by expressing and purifying the DNA polymerase gene of Thermophilic Geobacillus sp lacking the 5'→3' exonuclease domain in E.coli. It has 5'→3' DNA polymerase activity and strong strand displacement ability. It can be widely used in the rapid detection of pathogens based on isothermal amplification technology, single nucleotide polymorphism sequencing, rapid sequencing of GC-rich or ng-level DNA templates.

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Bst II Plus DNA Polymerase is derived from Thermophilic Geobacillus sp DNA Polymerase I, lacking 5'-3' exonuclease activity. Compared with Bst Ⅱ DNA Polymerase, the enzyme has stronger 5'-3' DNA polymerase activity, strand displacement activity and dUTP tolerance, which is more suitable for anti-pollution isothermal amplification reactions, such as LAMP, CPA, etc.

Applications

Suitable for a variety of isothermal amplification reactions such as LAMP, CPA, RCA, etc.

Activity Definition

1 U refers to the amount of enzyme required to incorporate 10 nmol of dNTP into the acid-insoluble precipitate in 30 min at 65°C.

Preservation Solution Components

10 mmol/L Tris-HCl, 50 mmol/L KCl, 0.1 mmol/L EDTA, 1 mmol/L DTT, 0.1% Triton X-100, 50% Glycerol, pH 7.5 @ 25℃.

Heat Inactivation

Incubation at 85°C for 5 min.

Shipping and Storage

The product is shipped with ice pack and can be stored at -20°C for 2 years. Please avoid repeated freeze-thaw.

Cautions

1. Bst Ⅱ Plus DNA Polymerase is sensitive to physical denaturation. Gently invert the tube when mixing, please do not shake vigorously.

2. Enzymes should be stored in an ice box or on an ice bath when used, and should be stored at -20°C immediately after use.

3. For your safety and health, please wear lab coats and disposable gloves for operation.