Uracil DNA Glycosylase (UDG/UNG), heat-labile, 1 U/μL

UDG enzyme, also known as Uracil DNA glycosylase, is an enzyme that catalyzes the hydrolysis of the N-glycosidic link between the uracil base and the sugar-phosphate backbone in ssDNA and dsDNA. It is usually used as an anti-pollution component of the PCR reaction system. Uracil DNA glycosylase (UDG/UNG) from Yeasen is a heat-labile purified recombinant protein expressed in E.coli. This UDG enzyme is featured with no nucleic acid endonuclease, exonuclease, and RNase residues.

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Product description

Heat-Labile UDG (uracil DNA glycosylase) catalyzes the release of free uracil from uracil-containing DNA by hydrolyzing the N-glycoside bond between uracil bases and sugar phosphate skeletons. Compared with conventional UDG enzyme, heat-Labile UDG can avoid the degradation of dU-containing amplification products caused by residual activity of the inactivated UDG at room temperature. Heat-Labile UDG (uracil DNA glycosylase) works at room temperature and is thermolabile and easy to be inactivated.

Product Applications

1. Remove aerosol pollution of dU-containing PCR products.

2. Remove uracil from single or double-stranded DNA.

Unit Definition

One unit (U) is defined as the amount of enzyme that required to catalyze the hydrolysis of 1 μg dU-containing dsDNA in 30 minutes at 25°C.

Heat Inactivation

50°C, 10 min.

Shipping and Storage

The product is shipped with dry ice and can be stored at -20°C for 2 years.

Product Notes

1. For your safety and health, please wear lab coats and disposable gloves for operation.

2. Heat-Labile UDG is active in most PCR reaction buffers.